Xu Zhiying, Xia Qing, Li Liu, Shi Yuxin, Gao Yuan, Ma Yichao, Liu Shu, He Yunhai, Wang Qiukuan, Ren Dandan
College of Food Science and Engineering, Dalian Ocean University, Dalian116023, China.
Xinjiang Standard Inspection Product Testing and Certification Co., Ltd., Urumqi 830000, China.
Foods. 2025 Apr 24;14(9):1486. doi: 10.3390/foods14091486.
Oligosaccharides possess characteristics such as low molecular weight, good solubility, and high bioavailability, which make them better absorbed than fucoidan. This study hypothesizes that fucoidan oligosaccharides can be absorbed by intestinal epithelial cells and quickly enter the bloodstream, with a rapid absorption rate. In this study, fucoidan oligosaccharides were obtained through acid degradation and Bio Gel column separation. By analyzing the chemical composition and molecular weight, oligosaccharides with smaller molecular weights and simpler monosaccharide compositions were selected for further research. A cell model and pharmacokinetic studies in mice were established to analyze the absorption patterns of the oligosaccharides. The results showed that after acid degradation and column separation, high-molecular-weight oligosaccharides SPF1 with a molecular weight range of 1.63 × 10 to 2.14 × 10 Da and the low-molecular-weight oligosaccharides SPF2 with a molecular weight range of 244.22 to 1545.36 Da were obtained. In cell transport and uptake experiments, the transport of SPF1 and SPF2 was positively correlated with time and negatively correlated with concentration. The transport rates of SPF1 and SPF2 ranged from 20% to 70%, with values greater than 1 × 10 cm/s. In the pharmacokinetics study, the blood concentration of the oligosaccharides in mice was simulated and analyzed using DAS 2.0, which indicated that the fucoidan oligosaccharides exhibited good absorption characteristics in vivo and in vitro. Therefore, fucoidan oligosaccharides with smaller molecular weights are more easily absorbed, which provides a theoretical basis for the application of fucoidan oligosaccharides.
低聚糖具有分子量低、溶解性好、生物利用度高等特点，这使得它们比岩藻多糖更容易被吸收。本研究假设岩藻多糖低聚糖可被肠道上皮细胞吸收并迅速进入血液循环，吸收速率较快。在本研究中，通过酸降解和生物凝胶柱分离获得岩藻多糖低聚糖。通过分析其化学组成和分子量，选择分子量较小、单糖组成较简单的低聚糖进行进一步研究。建立细胞模型和小鼠药代动力学研究以分析低聚糖的吸收模式。结果表明，经过酸降解和柱分离后，得到了分子量范围为1.63×10至2.14×10 Da的高分子量低聚糖SPF1和分子量范围为244.22至1545.36 Da的低分子量低聚糖SPF2。在细胞转运和摄取实验中，SPF1和SPF2的转运与时间呈正相关，与浓度呈负相关。SPF1和SPF2的转运速率在20%至70%之间， 值大于1×10 cm/s。在药代动力学研究中，使用DAS 2.0对小鼠体内低聚糖的血药浓度进行模拟分析，结果表明岩藻多糖低聚糖在体内外均表现出良好的吸收特性。因此，分子量较小的岩藻多糖低聚糖更容易被吸收，这为岩藻多糖低聚糖应用提供了理论依据。
