Trudel M, Séguin C, Nadon F, Boulay G, Trépanier P, Lussier G
Vet Microbiol. 1987 Feb;13(2):107-19. doi: 10.1016/0378-1135(87)90036-8.
The immunogenicity of bovine herpesvirus type 1 (BHV-1) hemagglutinin has been investigated. Both live and nonionic detergent solubilized vaccines were prepared and 5000 hemagglutinating units (HAU) were injected subcutaneously into rabbits. Both types of vaccine induced a good antibody response but live virus was four times more efficient in inducing hemagglutination inhibiting and neutralizing antibodies than either Triton X-100- or octylglucoside-solubilized subunit vaccine. Blotting analysis revealed that five proteins, of 105,000, 90,000, 74,000, 64,000 and 54,000 mol. wt, were recognized by the serum of vaccinated animals. Triton X-100-solubilized vaccine did not induce antibodies against the 105,000 and 64,000 mol. wt proteins, indicating the important role of VP 90,000 and VP 74,000 in hemagglutination and neutralization. The order in which antibodies to the different viral proteins were induced was VP 90,000, (VP 105,000, VP 64,000, VP 54,000) and VP 74,000. Our data indicate that VP 90,000 is the hemagglutinin. Using convalescent serum from intranasally infected animals, we could identify nine structural proteins for BHV-1; VP 105,000, VP 90,000, VP 74,000, VP 64,000, VP 54,000, VP 50,000, VP 47,000, VP 40,000 and VP 31,000.
对牛1型疱疹病毒（BHV - 1）血凝素的免疫原性进行了研究。制备了活疫苗和非离子去污剂溶解的疫苗，并将5000个血凝单位（HAU）皮下注射到兔子体内。两种类型的疫苗均诱导了良好的抗体反应，但活病毒在诱导血凝抑制和中和抗体方面的效率是Triton X - 100或辛基葡糖苷溶解的亚单位疫苗的四倍。印迹分析显示，接种动物血清可识别分子量为105,000、90,000、74,000、64,000和54,000的五种蛋白质。Triton X - 100溶解的疫苗未诱导针对分子量为105,000和64,000蛋白质的抗体，表明90,000道尔顿的病毒蛋白（VP 90,000）和74,000道尔顿的病毒蛋白（VP 74,000）在血凝和中和中起重要作用。诱导针对不同病毒蛋白的抗体的顺序为VP 90,000、（VP 105,000、VP 64,000、VP 54,000）和VP 74,000。我们的数据表明VP 90,000是血凝素。使用鼻内感染动物的恢复期血清，我们可以鉴定出BHV - 1的九种结构蛋白；VP 105,000、VP 90,000、VP 74,000、VP 64,000、VP 54,000、VP 50,000、VP 47,000、VP 40,000和VP 31,000。
