Trudel M, Nadon F, Seguin C, Boulay G, Lussier G
Centre de Recherche en Virologie, Institut Armand-Frappier, Université du Québec, Ville de Laval, Canada.
Vaccine. 1987 Sep;5(3):239-43. doi: 10.1016/0264-410x(87)90108-3.
Bovine herpesvirus type 1 has two major immunogenic surface glycoproteins: a 90 kDa haemagglutinin and the 130 (74 + 54) kDa glycoprotein. These proteins were purified by rate zonal sucrose density gradient centrifugation after extraction with nonionic detergent Triton X-100. For the preparation of ISCOMs, the glycoproteins were further adsorbed during a second rate zonal centrifugation, to micelles of Quil A glycoside, already added to the gradient. Haemagglutinating peaks were collected and used as subunit vaccine in rabbits: groups of three animals were injected with 50, 10 and 5 micrograms protein. Seroconversion was followed by ELISA, haemagglutination inhibition, neutralizing and plaque reduction assays. ISCOMs and unadsorbed subunits were efficient in inducing neutralizing as well as haemagglutination inhibiting antibodies: ISCOMs gave a higher level of response. The efficiency demonstrated by ISCOMs suggests their potential as a subunit vaccine.
牛疱疹病毒1型有两种主要的免疫原性表面糖蛋白：一种90 kDa的血凝素和130（74 + 54）kDa的糖蛋白。用非离子去污剂Triton X - 100提取后，通过速率区带蔗糖密度梯度离心法纯化这些蛋白质。为了制备免疫刺激复合物（ISCOM），在第二次速率区带离心过程中，将糖蛋白进一步吸附到已添加到梯度中的Quil A糖苷微胶粒上。收集血凝峰并将其用作兔的亚单位疫苗：每组三只动物分别注射50、10和5微克蛋白质。通过酶联免疫吸附测定（ELISA）、血凝抑制、中和及蚀斑减少试验来监测血清转化。免疫刺激复合物和未吸附的亚单位在诱导中和抗体以及血凝抑制抗体方面都很有效：免疫刺激复合物产生的反应水平更高。免疫刺激复合物所显示的有效性表明它们作为亚单位疫苗的潜力。
