College of Health Science, Wuhan Sports University, Wuhan, Hubei 430079, P.R. China.
Graduate School, Wuhan Sports University, Wuhan, Hubei 430079, P.R. China.
Int J Mol Med. 2018 Dec;42(6):3631-3639. doi: 10.3892/ijmm.2018.3922. Epub 2018 Oct 9.
Increasing evidence shows that repetitive transcranial magnetic stimulation (rTMS) promotes neurogenesis and the expression of microRNA (miR)‑106b. The present study investigated whether rTMS promotes the proliferation of neural progenitor cells (NPCs) and whether the effect is associated with the expression of miR‑106b. NPCs were cultured from the rat hippocampus and exposed to rTMS daily, comprising 1,000 stimuli for 3 days at 10 Hz, with 1.75 T output. The proliferation ability of the NPCs was revealed by EdU staining, and the levels of miR‑106b and downstream gene p21 in the NPCs were measured by reverse transcription‑quantitative polymerase chain reaction and western blot analyses. For analysis of the mechanism, the NPCs were transfected with Lenti‑miR‑106b or small interfering RNAs prior to rTMS. The results showed that: i) rTMS increased NPC proliferation, as revealed by the increased proportion of EdU‑positive cells; ii) rTMS was able to upregulate the expression of miR‑106b and downregulate the level of p21 in NPCs; iii) overexpression of miR‑106b further enhanced the effects of rTMS, whereas knockdown of miR‑106b had the opposite effects. Taken together, these data indicated that rTMS can promote NPC proliferation by upregulating the expression of miR‑106b and possibly inhibiting the expression of p21.
越来越多的证据表明，重复经颅磁刺激（rTMS）可促进神经发生和 microRNA（miR）-106b 的表达。本研究探讨了 rTMS 是否可促进神经祖细胞（NPC）的增殖，以及这种作用是否与 miR-106b 的表达有关。将 NPC 从大鼠海马中培养出来，并每天接受 rTMS 刺激，连续 3 天，每天 1000 个刺激，频率为 10Hz，输出为 1.75T。通过 EdU 染色揭示 NPC 的增殖能力，通过逆转录-定量聚合酶链反应和 Western blot 分析测量 NPC 中 miR-106b 和下游基因 p21 的水平。为了分析机制，在 rTMS 之前，将 NPC 用 Lenti-miR-106b 或小干扰 RNA 转染。结果表明：i）rTMS 增加了 NPC 的增殖，这表现为 EdU 阳性细胞的比例增加；ii）rTMS 能够上调 NPC 中 miR-106b 的表达，并下调 p21 的水平；iii）miR-106b 的过表达进一步增强了 rTMS 的作用，而 miR-106b 的敲低则产生相反的效果。综上所述，这些数据表明，rTMS 可以通过上调 miR-106b 的表达并可能抑制 p21 的表达来促进 NPC 的增殖。
