Gorelova N A, Krivánek J, Bures J
Brain Res. 1987 Feb 24;404(1-2):379-81. doi: 10.1016/0006-8993(87)91399-0.
Repetitive generation of spreading depression (SD) waves may induce metabolic and functional disturbances in the invaded brain regions. In order to better characterize this state, up to 60 SD waves were elicited in the cerebral cortex of anesthetized rats by intracortical injections of 2 microliters of isotonic K-acetate repeated at regular 5-min intervals. Propagation rate of SD between capillary electrodes located 1 mm and 7 mm from the injection site gradually decreased and after 35-45 waves became irregular. In another series of experiments SD-induced changes of cyclic adenosine monophosphate (cAMP) level were examined after 24 SD waves. Although no irregularities of SD propagation were observed, maximum level of cAMP in the SD-invaded cortex decreased from 270% in controls to 170% after 24 waves. Also, the recovery of the nucleotide was slowed down. Metabolic alterations thus preceded irregularities in SD propagation.
反复产生的扩散性抑制（SD）波可能会在受侵袭的脑区诱发代谢和功能紊乱。为了更好地表征这种状态，通过以5分钟的固定间隔重复皮层内注射2微升等渗醋酸钾，在麻醉大鼠的大脑皮层诱发多达60次SD波。位于距注射部位1毫米和7毫米处的毛细管电极之间的SD传播速率逐渐降低，在35 - 45次波后变得不规则。在另一系列实验中，在24次SD波后检查了SD诱导的环磷酸腺苷（cAMP）水平变化。尽管未观察到SD传播的不规则性，但受SD侵袭的皮层中cAMP的最高水平从对照组的270%降至24次波后的170%。此外，核苷酸的恢复也减慢了。因此，代谢改变先于SD传播的不规则性出现。
