Subedi Dinesh, Vijay Ajay Kumar, Willcox Mark
School of Optometry and Vision Science, University of New South Wales, Sydney, NSW 2052, Australia.
Antibiotics (Basel). 2018 Oct 15;7(4):88. doi: 10.3390/antibiotics7040088.
The prevalence of disinfectant resistance in is on the rise. is the most common bacteria isolated from cases of microbial keratitis. Many multi-purpose contact lens disinfectant solutions are available to decontaminate contact lenses before use and to help reduce the incidence of infections. However, with increasing disinfectant resistance, the effect of multi-purpose disinfectant solutions may diminish. The goal of this study was to examine genes associated with disinfectant resistance in ocular isolates of and understand the strain's susceptibility to different multipurpose disinfectant solutions.
Seven potential disinfectant resistance genes were used in BLASTn searches against the whole genomes of 13 eye isolates of . A microdilution broth method was used to examine susceptibility to four different multipurpose disinfectant solutions.
All strains possessed the , and () genes. The and genes were present in 6/13 isolates. No strains contained the or genes. All tested disinfectant solutions had the ability to kill all test strains at 100% concentration, with some strains being susceptible at 1:8 dilutions of the disinfecting solutions. However, the presence of disinfectant resistance genes was not associated with susceptibility to multi-purpose disinfectants.
All four tested contact lens disinfectant preparations are effective against isolates regardless of the presence of disinfectant resistance genes.
[细菌名称]中消毒剂耐药性的发生率正在上升。[细菌名称]是从微生物性角膜炎病例中分离出的最常见细菌。许多多功能隐形眼镜消毒溶液可在使用前对隐形眼镜进行消毒，并有助于降低感染发生率。然而，随着消毒剂耐药性的增加，多功能消毒溶液的效果可能会减弱。本研究的目的是检查[细菌名称]眼部分离株中与消毒剂耐药性相关的基因，并了解该菌株对不同多功能消毒溶液的敏感性。
使用七个潜在的消毒剂耐药基因对13株[细菌名称]眼部分离株的全基因组进行BLASTn搜索。采用微量稀释肉汤法检测对四种不同多功能消毒溶液的敏感性。
所有菌株均具有[基因名称1]、[基因名称2]和[基因名称3]（[基因缩写]）基因。[基因名称4]和[基因名称5]基因存在于6/13的分离株中。没有菌株含有[基因名称6]或[基因名称7]基因。所有测试的消毒溶液在100%浓度下都有能力杀死所有测试菌株，一些菌株在消毒溶液1:8稀释时敏感。然而，消毒剂耐药基因的存在与对多功能消毒剂的敏感性无关。
所有四种测试的隐形眼镜消毒制剂对[细菌名称]分离株均有效，无论是否存在消毒剂耐药基因。
