小鼠6号染色体上的内源性逆转录病毒Mtv-8定位在几个κ轻链标记附近。

The endogenous retrovirus Mtv-8 on mouse chromosome 6 maps near several kappa light chain markers.

作者信息

Yang J N, Boyd R T, Gottlieb P D, Dudley J P

出版信息

Immunogenetics. 1987;25(4):222-7. doi: 10.1007/BF00404691.

Abstract

Endogenous retroviruses are known to affect expression of cellular genes in the vicinity of their integration sites. The endogenous mouse mammary tumor provirus (Mtv-8) previously has been reported to reside on mouse chromosome 6 near the immunoglobulin kappa chain locus. Using pairs of mouse strains on the BALB/c (Mtv-8 positive) and C58 (Mtv-8 negative) backgrounds which are congenic for chromosome 6 genetic markers, we have confirmed the chromosome assignment of this provirus. Moreover, we have analyzed the N1 progeny of a (B6 X C58) X C58 backcross to determine the segregation of the Mtv-8 provirus with respect to polymorphisms in the Igk-VSer and Igk-J loci. The results with congenic and backcross mice together with results of others suggest that Mtv-8 is located approximately 0.52 cM from several closely linked kappa markers on chromosome 6.

摘要

已知内源性逆转录病毒会影响其整合位点附近细胞基因的表达。内源性小鼠乳腺肿瘤前病毒（Mtv - 8）此前已有报道称位于小鼠6号染色体上靠近免疫球蛋白κ链基因座的位置。利用在BALB/c（Mtv - 8阳性）和C58（Mtv - 8阴性）背景下针对6号染色体遗传标记同基因的小鼠品系对，我们已经证实了这种前病毒的染色体定位。此外，我们分析了（B6×C58）×C58回交的N1子代，以确定Mtv - 8前病毒相对于Igk - VSer和Igk - J基因座多态性的分离情况。同基因和回交小鼠的结果以及其他研究结果表明，Mtv - 8位于6号染色体上与几个紧密连锁的κ标记相距约0.52厘摩的位置。