编码小鼠免疫球蛋白重链的结构基因簇的染色体定位。
Chromosomal location of the structural gene cluster encoding murine immunoglobulin heavy chains.
作者信息
D'Eustachio P, Pravtcheva D, Marcu K, Ruddle F H
出版信息
J Exp Med. 1980 Jun 1;151(6):1545-50. doi: 10.1084/jem.151.6.1545.
Abstract
To determine the chromosomal location of mouse immunoglobulin heavy chain structural genes unambiguously, a panel of somatic cell hybrids was scored for the presence of DNA sequences homologous to gamma 2b-, mu-, and alpha-heavy chain-constant region DNA probe molecules. The hybrids, formed between mouse and hamster cells, contained various combinations of mouse chromosomes plus a full set of hamster chromosomes. Hybrids that retained mouse chromosome 12 reacted with the probes, whereas hybrids that had lost the chromosome, or its distal half, failed to react. These results indicate that structural genes for the gamma 2b-, mu-, and alpha-heavy chain-constant regions map to the distal half of this chromosome.
摘要
为了明确确定小鼠免疫球蛋白重链结构基因的染色体定位，对一组体细胞杂种进行了检测，以寻找与γ2b、μ和α重链恒定区DNA探针分子同源的DNA序列。这些杂种由小鼠细胞和仓鼠细胞形成，包含小鼠染色体的各种组合以及一整套仓鼠染色体。保留小鼠12号染色体的杂种与探针发生反应，而丢失该染色体或其远端一半的杂种则不发生反应。这些结果表明，γ2b、μ和α重链恒定区的结构基因定位于该染色体的远端一半。
相似文献
1
Chromosomal location of the structural gene cluster encoding murine immunoglobulin heavy chains.编码小鼠免疫球蛋白重链的结构基因簇的染色体定位。
J Exp Med. 1980 Jun 1;151(6):1545-50. doi: 10.1084/jem.151.6.1545.
2
Deletions in the constant region locus can account for switches in immunoglobulin heavy chain expression.恒定区基因座的缺失可解释免疫球蛋白重链表达的转换。
Nature. 1980 Jun 12;285(5765):450-6. doi: 10.1038/285450a0.
3
4
Chromosomal locations of mouse immunoglobulin genes.小鼠免疫球蛋白基因的染色体定位。
Proc Natl Acad Sci U S A. 1978 Jun;75(6):2883-7. doi: 10.1073/pnas.75.6.2883.
5
Rearrangement of immunoglobulin heavy chain genes during B-lymphocyte development as revealed by studies of mouse plasmacytoma cells.通过对小鼠浆细胞瘤细胞的研究揭示B淋巴细胞发育过程中免疫球蛋白重链基因的重排。
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1422-6. doi: 10.1073/pnas.77.3.1422.
6
Deletion of immunoglobulin heavy chain genes from expressed allelic chromosome.从表达的等位染色体上删除免疫球蛋白重链基因。
Nature. 1980 Aug 28;286(5776):850-3. doi: 10.1038/286850a0.
7
Arrangement of human immunoglobulin heavy chain constant region genes implies evolutionary duplication of a segment containing gamma, epsilon and alpha genes.人类免疫球蛋白重链恒定区基因的排列意味着包含γ、ε和α基因的片段发生了进化性重复。
Nature. 1982 Dec 23;300(5894):709-13. doi: 10.1038/300709a0.
8
The role of gene deletion in the immunoglobulin heavy chain switch.基因缺失在免疫球蛋白重链转换中的作用。
Nature. 1980 Jan 24;283(5745):351-6. doi: 10.1038/283351a0.
9
Exon shuffling generates an immunoglobulin heavy chain gene.外显子改组产生免疫球蛋白重链基因。
Proc Natl Acad Sci U S A. 1980 Apr;77(4):2138-42. doi: 10.1073/pnas.77.4.2138.
10
Mapping of the genes for mouse immunoglobulins M and D.小鼠免疫球蛋白M和D基因的定位
Crit Rev Immunol. 1983;4(2):169-95.
引用本文的文献
1
Fusion of DNA region to murine immunoglobulin heavy chain locus corresponds to plasmacytoma-associated chromosome translocation.DNA区域与小鼠免疫球蛋白重链基因座的融合对应于浆细胞瘤相关的染色体易位。
EMBO J. 1983;2(2):213-6. doi: 10.1002/j.1460-2075.1983.tb01407.x.
2
Effect of the mouse scid mutation on meiotic recombination.
Mamm Genome. 1996 Jul;7(7):497-500. doi: 10.1007/s003359900150.
3
Chromosome assignment of the tumor-specific antigen of a 3-methylcholanthrene-induced mouse sarcoma.3-甲基胆蒽诱导的小鼠肉瘤肿瘤特异性抗原的染色体定位
J Exp Med. 1981 Sep 1;154(3):964-77. doi: 10.1084/jem.154.3.964.
4
Structural genes of the mouse major urinary protein are on chromosome 4.小鼠主要尿蛋白的结构基因位于4号染色体上。
J Cell Biol. 1982 Aug;94(2):414-7. doi: 10.1083/jcb.94.2.414.
5
Autoimmune diseases: immunopathology and etiopathogenesis.自身免疫性疾病：免疫病理学与病因发病机制
Am J Pathol. 1982 Sep;108(3):319-65.
6
DNA sequence associated with chromosome translocations in mouse plasmacytomas.与小鼠浆细胞瘤中染色体易位相关的DNA序列。
Proc Natl Acad Sci U S A. 1982 Nov;79(21):6622-6. doi: 10.1073/pnas.79.21.6622.
7
The genes coding for the muscle contractile proteins, myosin heavy chain, myosin light chain 2, and skeletal muscle actin are located on three different mouse chromosomes.编码肌肉收缩蛋白、肌球蛋白重链、肌球蛋白轻链2和骨骼肌肌动蛋白的基因位于三条不同的小鼠染色体上。
EMBO J. 1982;1(11):1299-305. doi: 10.1002/j.1460-2075.1982.tb01314.x.
8
Characterization of superoxide dismutase (SOD-1 and SOD-2) activities in inbred mice: evidence for quantitative variability and possible nonallelic SOD-1 polymorphism.近交系小鼠中超氧化物歧化酶（SOD-1和SOD-2）活性的特征：数量变异性及可能的非等位基因SOD-1多态性的证据
Biochem Genet. 1983 Apr;21(3-4):349-64. doi: 10.1007/BF00499144.
9
Chromosomal location of human kappa and lambda immunoglobulin light chain constant region genes.人类κ和λ免疫球蛋白轻链恒定区基因的染色体定位。
J Exp Med. 1982 May 1;155(5):1480-90. doi: 10.1084/jem.155.5.1480.
10
The William Allan Memorial Award address: Reverse genetics and beyond.威廉·艾伦纪念奖演讲：反向遗传学及其他
Am J Hum Genet. 1984 Sep;36(5):944-53.
本文引用的文献
1
Linkage analyses of murine immunoglobulin heavy chain and serum prealbumin genes establish their location on chromosome 12 proximal to the T (5;12) 31H breakpoint in band 12F1.对小鼠免疫球蛋白重链基因和血清前白蛋白基因的连锁分析确定了它们在12号染色体上的位置，该位置靠近12F1带中的T(5;12)31H断点。
Proc Natl Acad Sci U S A. 1980 Jan;77(1):550-3. doi: 10.1073/pnas.77.1.550.
2
Congenic mouse strains: the expression of a hidden immunoglobulin allotype in a congenic partner strain of BALB-c mice.同源近交系小鼠：BALB-c小鼠同源近交系伙伴中隐藏免疫球蛋白同种异型的表达。
J Exp Med. 1974 Mar 1;139(3):512-27. doi: 10.1084/jem.139.3.512.
3
A system of nomenclature for band patterns of mouse chromosomes.小鼠染色体带型命名系统。
Chromosoma. 1973;41(2):145-58. doi: 10.1007/BF00319691.
4
Nucleotide sequence of the rightward operator of phage lambda.噬菌体λ右向操纵基因的核苷酸序列。
Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8. doi: 10.1073/pnas.72.3.1184.
5
Isolation and characterization of IgG molecules expressing latent group b allotypes from pedigreed b4b4 rabbits.从纯种b4b4兔中分离并鉴定表达潜在B组同种异型的IgG分子。
J Exp Med. 1978 Aug 1;148(2):522-33. doi: 10.1084/jem.148.2.522.
6
Analysis of the beta-delta-globin gene loci in normal and Hb Lepore DNA: direct determination of gene linkage and intergene distance.正常和血红蛋白Lepore DNA中β-δ-珠蛋白基因位点的分析：基因连锁和基因间距离的直接测定
Cell. 1978 Sep;15(1):25-41. doi: 10.1016/0092-8674(78)90080-6.
7
8
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.通过使用硫酸葡聚糖将大的DNA片段从琼脂糖凝胶高效转移至重氮苄氧基甲基纸并进行快速杂交。
Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7. doi: 10.1073/pnas.76.8.3683.
9
Stable association of the human transgenome and host murine chromosomes demonstrated with trispecific microcell hybrids.利用三特异性微细胞杂种证明人类转基因组与宿主小鼠染色体的稳定关联。
Proc Natl Acad Sci U S A. 1977 Sep;74(9):3937-41. doi: 10.1073/pnas.74.9.3937.
10
Molecular analysis of chromosome-mediated gene transfer.染色体介导的基因转移的分子分析
Proc Natl Acad Sci U S A. 1979 Aug;76(8):3987-90. doi: 10.1073/pnas.76.8.3987.
Zotero 插件