Gottlieb P D, Tsang H C, Gibson D M, Cannon L E
Proc Natl Acad Sci U S A. 1981 Jan;78(1):559-63. doi: 10.1073/pnas.78.1.559.
The C.C58 and C.AKR congeneic strains of mice differ from BALB/c at loci on chromosome 6 which govern kappa light chain variable region (V kappa) polymorphisms and the Lyt-2 and Lyt-3 alloantigens. Amino acid sequence analysis of light chains of myelomas induced in these strains revealed one light chain, C.C58 M75, that had an NH2-terminal serine and differed sufficiently from published V kappa sequences to define a new V kappa group, V kappa (Ser), apparently not expressed by BALB/c mice. Peptide map analysis indicated that the M75 light chain contained the IB-peptide marker, a V kappa polymorphism expressed by C.C58 but not BALB/c mice, which is determined by the IgK-Trpa allele present on chromosome 6. This same light chain was found by isoelectric focussing to correspond to IgK-Ef1a, another V kappa genetic marker of C.C58 and C.AKR. Isoelectric focussing of approximately 200 C.C58 and C.AKR myeloma light chains revealed three additional C.C58 and four C.AKR light chains that corresponded to IgK-Ef1a-specific light chains. All three additional C.C58 light chains belonged to the V kappa (Ser) group and contained the IB-peptide marker. Thus, the differences in V kappa repertoires represented by the IB-peptide and IgK-Ef1a markers and controlled by genes on chromosome 6 appear to reflect expression (or failure of expression) of a distinct group of V kappa regions.
C.C58和C.AKR同基因系小鼠在6号染色体上的位点与BALB/c小鼠不同，这些位点控制κ轻链可变区（Vκ）多态性以及Lyt-2和Lyt-3同种抗原。对这些品系中诱导产生的骨髓瘤轻链进行氨基酸序列分析，发现一条轻链C.C58 M75，其NH2末端为丝氨酸，与已发表的Vκ序列差异足够大，从而定义了一个新的Vκ组，即Vκ(Ser)，显然BALB/c小鼠不表达该组。肽图分析表明，M75轻链含有IB肽标记，这是一种由C.C58而非BALB/c小鼠表达的Vκ多态性，由6号染色体上存在的IgK-Trpa等位基因决定。通过等电聚焦发现，这条相同的轻链对应于IgK-Ef1a，这是C.C58和C.AKR的另一个Vκ遗传标记。对大约200条C.C58和C.AKR骨髓瘤轻链进行等电聚焦分析，发现另外三条C.C58轻链和四条C.AKR轻链对应于IgK-Ef1a特异性轻链。所有另外三条C.C58轻链都属于Vκ(Ser)组，并含有IB肽标记。因此，由IB肽和IgK-Ef1a标记所代表的、受6号染色体上基因控制的Vκ库差异，似乎反映了一组不同Vκ区域的表达（或不表达）情况。
