Morozova Olga B, Ivanov Konstantin L
International Tomography Center, Institutskaya 3a, 630090, Novosibirsk, Russia.
Novosibirsk State University, Pirogova 2, 630090, Novosibirsk, Russia.
Chemphyschem. 2019 Jan 21;20(2):197-215. doi: 10.1002/cphc.201800566. Epub 2018 Nov 28.
In this work, we review the hyperpolarization technique named chemically induced dynamic nuclear polarization (CIDNP), focusing on the time-resolved variant of this method and its biological applications. We introduce the main principles of polarization formation in liquids at high magnetic fields, provided by the so-called spin sorting mechanism. Applications of CIDNP to studying fast reactions of short-lived free radicals of biologically important molecules are discussed, as well as the potential of the method to probe the structure and magnetic parameters of such radicals. We also explain the principles of protein CIDNP and discuss applications of time-resolved CIDNP to studies of protein structure and dynamics.
在这项工作中，我们回顾了名为化学诱导动态核极化（CIDNP）的超极化技术，重点关注该方法的时间分辨变体及其生物学应用。我们介绍了在高磁场下液体中极化形成的主要原理，这是由所谓的自旋分选机制提供的。讨论了CIDNP在研究具有生物学重要性的分子的短寿命自由基的快速反应中的应用，以及该方法探测此类自由基的结构和磁参数的潜力。我们还解释了蛋白质CIDNP的原理，并讨论了时间分辨CIDNP在蛋白质结构和动力学研究中的应用。
