da Costa Santos Vanessa Batista, de Paula Ramos Solange, Milanez Vinícius Flávio, Corrêa Julio Cesar Molina, de Andrade Alves Rubens Igor, Dias Ivan Frederico Lupiano, Nakamura Fábio Yuzo
Department of Physical Education, Universidade Estadual de Londrina, Londrina, Paraná, Brazil.
Lasers Med Sci. 2014 Mar;29(2):599-605. doi: 10.1007/s10103-013-1371-9. Epub 2013 Jun 19.
The aim of this study was to test, between two bouts of exercise, the effects of light-emitting diode (LED) therapy and cryotherapy regarding muscle damage, inflammation, and performance. Male Wistar rats were allocated in four groups: control, passive recovery (PR), cryotherapy (Cryo), and LED therapy. The animals were submitted to 45 min of swimming exercise followed by 25 min of recovery and then a second bout of either 45 min of exercise (muscle damage analysis) or time to exhaustion (performance). During the rest intervals, the rats were kept in passive rest (PR), submitted to cold water immersion (10 min, 10 °C) or LED therapy (940 nm, 4 J/cm(2)) of the gastrocnemius muscle. Blood samples were collected to analyze creatine kinase activity (CK), C-reactive protein (CRP), and leukocyte counts. The soleus muscles were evaluated histologically. Time to exhaustion was recorded during the second bout of exercise. After a second bout of 45 min, the results demonstrated leukocytosis in the PR and Cryo groups. Neutrophil counts were increased in all test groups. CK levels were increased in the Cryo group. CRP was increased in PR animals. The PR group presented a high frequency of necrosis, but the LED group had fewer necrotic areas. Edema formation was prevented, and fewer areas of inflammatory cells were observed in the LED group. The time to exhaustion was greater in both the LED and Cryo groups, without differences in CK levels. CRP was decreased in LED animals. We conclude that LED therapy and cryotherapy can improve performance, although LED therapy is more efficient in preventing muscle damage and local and systemic inflammation.
本研究的目的是测试在两次运动之间，发光二极管（LED）疗法和冷冻疗法对肌肉损伤、炎症和运动表现的影响。将雄性Wistar大鼠分为四组：对照组、被动恢复组（PR）、冷冻疗法组（Cryo）和LED疗法组。动物先进行45分钟的游泳运动，随后休息25分钟，然后进行第二轮45分钟的运动（肌肉损伤分析）或直至力竭（运动表现）。在休息期间，大鼠处于被动休息状态（PR），或对腓肠肌进行冷水浸泡（10分钟，10°C）或LED疗法（940纳米，4焦耳/平方厘米）。采集血样以分析肌酸激酶活性（CK）、C反应蛋白（CRP）和白细胞计数。对比目鱼肌进行组织学评估。在第二轮运动期间记录力竭时间。在进行第二轮45分钟运动后，结果显示PR组和Cryo组出现白细胞增多。所有测试组的中性粒细胞计数均增加。Cryo组的CK水平升高。PR组动物的CRP升高。PR组出现坏死的频率较高，但LED组的坏死区域较少。LED组预防了水肿形成，且观察到的炎症细胞区域较少。LED组和Cryo组的力竭时间均延长，CK水平无差异。LED组动物的CRP降低。我们得出结论，LED疗法和冷冻疗法均可改善运动表现，尽管LED疗法在预防肌肉损伤以及局部和全身炎症方面更有效。
