Nagle Michael, Déjardin Annabelle, Pilate Gilles, Strauss Steven H
Forest Ecosystems and Society, Molecular and Cellular Biology, Oregon State University, Corvallis, OR, United States.
BioForA, INRA, ONF, Orléans, France.
Front Plant Sci. 2018 Oct 2;9:1443. doi: 10.3389/fpls.2018.01443. eCollection 2018.
The incorporation of DNA into plant genomes followed by regeneration of non-chimeric stable plants (transformation) remains a major challenge for most plant species. Forest trees are particularly difficult as a result of their biochemistry, aging, desire for clonal fidelity, delayed reproduction, and high diversity. We review two complementary approaches to transformation that appear to hold promise for forest trees.
将DNA整合到植物基因组中，随后再生出非嵌合稳定植株（转化），对大多数植物物种而言仍是一项重大挑战。由于林木的生物化学特性、老化、对克隆保真度的要求、繁殖延迟以及高度多样性，对其进行转化尤为困难。我们综述了两种互补的转化方法，它们似乎对林木具有应用前景。
