Ruggerone Beatrice, Giraldi Marco, Paltrinieri Saverio, Scarpa Paola
Department of Veterinary Medicine, University of Milan, Milan, Italy.
Veterinary Teaching Hospital, University of Milan, Lodi, Italy.
Vet Clin Pathol. 2018 Dec;47(4):617-624. doi: 10.1111/vcp.12665. Epub 2018 Oct 18.
Several breeds have physiological peculiarities that induce variations in reference intervals (RIs) compared with the general canine population. Shetland sheepdogs (SSs) are reported to be more predisposed to different diseases (eg, hyperlipidemia, gallbladder mucocele, and hypothyroidism). Consequently, a breed-specific approach is more often required.
The aim of this study was to determine whether the RIs of the general canine population could be applied to that of SSs, and to generate breed-specific RIs, where appropriate.
Sixty clinically healthy and fasted SSs (36% of the population registered at the Italian Breed association) were examined. Routine hematology and biochemistry analyses were performed. The transference method was used to compare the results of SSs with the RIs of the general canine population. When these RIs were not validated, new RIs were generated according to the guidelines of the American Society of Veterinary Clinical Pathology. Differences associated with sex, age, coat color, and whether used as a pet, a herding dog, or an agility dog were also investigated.
The transference method validated for 30/38 SS RIs. For 6 of the remaining 8 variables, the difference with the claimed RIs could depend on preanalytical or analytical artifacts, whereas for glucose and total cholesterol, these differences could depend on breed peculiarities. However, in all SSs, the concentration of cholesterol was <12.95 mmol/L. Relevant differences associated with sex, age, coat color, and use were not found.
This study suggests that breed-specific RIs should be used for glucose and cholesterol in SSs.
与一般犬类群体相比，有几个品种具有生理特性，会导致参考区间（RI）出现差异。据报道，设得兰牧羊犬（SS）更容易患不同疾病（如高脂血症、胆囊黏液囊肿和甲状腺功能减退）。因此，通常需要采用特定品种的方法。
本研究的目的是确定一般犬类群体的RI是否适用于SS，并在适当情况下生成特定品种的RI。
对60只临床健康且禁食的SS（占意大利品种协会注册犬只总数的36%）进行了检查。进行了常规血液学和生化分析。采用转移法将SS的结果与一般犬类群体的RI进行比较。当这些RI未经验证时，根据美国兽医临床病理学会的指南生成新的RI。还研究了与性别、年龄、毛色以及是否作为宠物、牧羊犬或敏捷犬使用相关的差异。
转移法验证了38个SS RI中的30个。对于其余8个变量中的6个，与声称的RI的差异可能取决于分析前或分析过程中的假象，而对于葡萄糖和总胆固醇，这些差异可能取决于品种特性。然而，在所有SS中，胆固醇浓度均<12.95 mmol/L。未发现与性别、年龄、毛色和用途相关的显著差异。
本研究表明，SS的葡萄糖和胆固醇应使用特定品种的RI。
