Department of Virology, Veterinary Research Institute, Brno, Czech Republic.
Department of Virology, Veterinary Research Institute, Brno, Czech Republic; Institute of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic.
Vaccine. 2018 Nov 19;36(48):7257-7261. doi: 10.1016/j.vaccine.2018.10.034. Epub 2018 Oct 15.
In tick-borne encephalitis (TBE) endemic areas, consumption of unpasteurized milk or milk products from grazing domestic ruminants (goats, cattle, and sheep) represents a risk of TBE virus (TBEV) infection for humans. In addition to vaccination of humans, human alimentary TBEV infections can be avoided by pasteurizing milk or by vaccination of the ruminants. However, there is presently no TBEV vaccine for veterinary use. Here, we developed a new veterinary TBE vaccine candidate based on cell culture-derived, purified, and formaldehyde-inactivated TBEV (strain Hypr). The safety and immunogenicity of the vaccine was evaluated in mice and sheep and was well-tolerated while eliciting the production of high levels of virus-neutralizing antibodies. Vaccination provided full protection against lethal TBE in mice, prevented development of viremia in sheep and presence of TBEV in milk of lactating ewes. This vaccine is a good candidate for immunization of ruminants to prevent alimentary milk-borne TBEV infections in humans.
在蜱传脑炎（TBE）流行地区，食用未经巴氏消毒的牛奶或来自放牧的家养反刍动物（山羊、牛和绵羊）的奶制品会使人类感染 TBE 病毒（TBEV）。除了对人类进行疫苗接种外，还可以通过巴氏消毒牛奶或对反刍动物进行疫苗接种来避免人类食源性 TBEV 感染。然而，目前尚无兽用 TBEV 疫苗。在这里，我们基于细胞培养的、纯化的和甲醛失活的 TBEV（株 Hypr）开发了一种新的兽用 TBE 疫苗候选物。该疫苗在小鼠和绵羊中的安全性和免疫原性进行了评估，耐受性良好，同时诱导产生高水平的病毒中和抗体。疫苗接种使小鼠完全免受致死性 TBE 的侵害，防止绵羊发生病毒血症，并防止哺乳期母羊的牛奶中存在 TBEV。该疫苗是一种很好的候选物，可用于对反刍动物进行免疫接种，以防止人类食源性 TBEV 感染。
