Department of Hematology and Laboratory Sciences, Faculty of Allied Medical Sciences, Kerman University of Medical Sciences, Kerman, Iran; Cell Therapy and Regenerative Medicine Comprehensive Center, Kerman University of Medical Sciences, Kerman, Iran.
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Int J Biol Macromol. 2023 Jun 1;239:124099. doi: 10.1016/j.ijbiomac.2023.124099. Epub 2023 Mar 21.
Wound dressing is applied to promote the healing process, wound protection, and additionally regeneration of injured skin. In this study, a bilayer scaffold composed of a hydrogel and nanofibers was fabricated to improve the regeneration of injured skin. To this end, polycaprolactone/gelatin (PCL/Gel) nanofibers were electrospun directly on the prepared collagen/alginate (Col/Alg) hydrogel. The bilayer scaffold was characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR), mechanical properties, and swelling/degradation time. Cytotoxicity assays were evaluated using MTT assay. Then, the nanofiber and bilayer scaffolds were seeded with Adipose-derived stem cells (ADSCs). ADSCs were isolated from rat adipose tissue and analyzed using flow cytometry, in advance. Full-thickness wounds on the backs of rats were dressed with ADSCs-seeded bilayer scaffolds and nanofibers. Histopathological evaluations were performed after 14 and 21 days using H&E (hematoxylin and eosin) staining. The results indicated that re-epithelialization, angiogenesis, and collagen remodeling were enhanced in ADSCs-seeded bilayer scaffolds and nanofibers in comparison with the control group. In conclusion, the best re-epithelialization, collagen organization, neovascularization, and low presence of inflammation in the wound area were observed in the ADSCs-seeded bilayer scaffolds.
伤口敷料的应用旨在促进愈合过程、保护伤口，并促进受伤皮肤的再生。在本研究中，制备了一种由水凝胶和纳米纤维组成的双层支架，以改善受伤皮肤的再生。为此，将聚己内酯/明胶（PCL/Gel）纳米纤维直接电纺在制备好的胶原/海藻酸钠（Col/Alg）水凝胶上。通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）、机械性能和溶胀/降解时间对双层支架进行了表征。通过 MTT 测定法评估了细胞毒性试验。然后，将纳米纤维和双层支架接种脂肪来源干细胞（ADSCs）。预先使用流式细胞术从大鼠脂肪组织中分离 ADSCs。用 ADSCs 接种的双层支架和纳米纤维对大鼠背部的全层伤口进行包扎。通过 H&E（苏木精和伊红）染色，在 14 天和 21 天后进行组织病理学评估。结果表明，与对照组相比，双层支架和纳米纤维中 ADSCs 的接种促进了再上皮化、血管生成和胶原重塑。总之，在 ADSCs 接种的双层支架中，观察到伤口区域的再上皮化、胶原组织、新生血管形成和炎症的低发生率最佳。
