Sokół Ireneusz, Gaweł Andrzej, Bobrek Kamila
A Private Veterinary Service, Agencja Handlu Drobiem i Mięsem sp. z o.o. ul Sikorskiego 17/6, 65-454 Zielona, Góra, Poland.
B Department of Epizootiology with Clinic of Bird and Exotic Animals, Wroclaw University of Environmental and Life Sciences, pl. Grunwaldzki, 45 50-366 Wrocław, Poland.
Avian Dis. 2018 Sep;62(3):286-290. doi: 10.1637/11780-121117-Reg.1.
This study was undertaken to explore the issue of yeast species prevalence in colonizing the gastrointestinal tract of healthy turkeys. The samples were collected from the beak cavity, crop, and cloaca of 5-wk-old turkeys, cultured and classified using morphological, biochemical, and genetic analysis based on ITS1-5.8rRNA-ITS2 fragment sequencing. Out of all the samples, 12.4% were yeast positive. The highest number of strains, 50% of the total collected, were isolated from the crop, with 30.8% coming from the beak cavity and 19.2% from the cloaca. The most frequently isolated yeast belonged to Candida species (88.5%), followed by Trichosporon (7.7%) and Rhodotorula (3.8%). The most prevalent species was Candida catenulata (30.7%), followed by Candida albicans (21.7%), Candida palmioleophila (17.4%), Candida rugosa (17.4%), and Candida glabrata (8.7%). The present study, showing the prevalence of yeast species of the gastrointestinal tract, is a stepping stone to investigating the physiological mycobiota of turkeys' gastrointestinal tract.
本研究旨在探讨酵母菌种在健康火鸡胃肠道定殖中的流行问题。样本取自5周龄火鸡的喙腔、嗉囊和泄殖腔，基于ITS1-5.8rRNA-ITS2片段测序，通过形态学、生化和遗传学分析进行培养和分类。在所有样本中，12.4%的样本酵母呈阳性。分离出的菌株数量最多，占总采集量的50%，来自嗉囊，30.8%来自喙腔，19.2%来自泄殖腔。最常分离出的酵母属于念珠菌属（88.5%），其次是丝孢酵母属（7.7%）和红酵母属（3.8%）。最常见的菌种是链状念珠菌（30.7%），其次是白色念珠菌（21.7%）、嗜棕榈念珠菌（17.4%）、皱落念珠菌（17.4%）和光滑念珠菌（8.7%）。本研究显示了火鸡胃肠道酵母菌种的流行情况，是研究火鸡胃肠道生理性真菌群的垫脚石。
