Department of Molecular Biology, Princeton University, Lewis Thomas Laboratory, Washington Road, Princeton, NJ 08544, USA.
Department of Molecular Biology, Princeton University, Lewis Thomas Laboratory, Washington Road, Princeton, NJ 08544, USA.
Curr Opin Chem Biol. 2019 Feb;48:34-43. doi: 10.1016/j.cbpa.2018.09.021. Epub 2018 Oct 16.
Protein movement between different subcellular compartments is an essential aspect of biological processes, including transcriptional and metabolic regulation, and immune and stress responses. As obligate intracellular parasites, viruses are master manipulators of cellular composition and organization. Accumulating evidences have highlighted the importance of infection-induced protein translocations between organelles. Both directional and temporal, these translocation events facilitate localization-dependent protein interactions and changes in protein functions that contribute to either host defense or virus replication. The discovery and characterization of protein movement is technically challenging, given the necessity for sensitive detection and subcellular resolution. Here, we discuss infection-induced translocations of host and viral proteins, and the value of integrating quantitative proteomics with advanced microscopy for understanding the biology of human virus infections.
蛋白质在不同亚细胞区室之间的运动是生物过程的一个重要方面，包括转录和代谢调节、免疫和应激反应。作为专性细胞内寄生虫，病毒是细胞成分和组织的主要操纵者。越来越多的证据强调了感染诱导的细胞器间蛋白质易位的重要性。这些易位事件具有方向性和时间性，有利于定位依赖性蛋白质相互作用和蛋白质功能的变化，有助于宿主防御或病毒复制。鉴于需要敏感检测和亚细胞分辨率，发现和描述蛋白质运动在技术上具有挑战性。在这里，我们讨论了感染诱导的宿主和病毒蛋白的易位，以及将定量蛋白质组学与先进显微镜技术相结合用于理解人类病毒感染生物学的价值。
