Donaldson K, Slight J, Bolton R E
Agents Actions. 1987 Feb;20(1-2):87-92. doi: 10.1007/BF01965629.
Rat alvolar macrophages demonstrated plasminogen dependent fibrinolysis in vitro which was inhibited to varying degrees by the addition of zymosan, the non-toxic particulate titanium dioxide, and the toxic dusts quartz and chrysotile asbestos. Assessment of viability suggested that the inhibition produced by zymosan and titanium dioxide could be accounted for by cytotoxic effects but in the case of quartz and chrysotile asbestos there was evidence that stimulation of fibrinolysis preceded cell death. Zymosan, which caused no observeable enhancement of alveolar macrophage fibrinolysis was found to markedly stimulate peritoneal macrophage fibrinolysis. The choice of assays of cell function to assess the action of toxic dusts are discussed.
大鼠肺泡巨噬细胞在体外表现出纤溶酶原依赖性纤维蛋白溶解，添加酵母聚糖、无毒颗粒二氧化钛以及有毒粉尘石英和温石棉后，该过程受到不同程度的抑制。活力评估表明，酵母聚糖和二氧化钛产生的抑制作用可归因于细胞毒性效应，但对于石英和温石棉，有证据表明在细胞死亡之前纤维蛋白溶解就已受到刺激。未观察到酵母聚糖增强肺泡巨噬细胞纤维蛋白溶解，但发现它能显著刺激腹腔巨噬细胞纤维蛋白溶解。本文讨论了用于评估有毒粉尘作用的细胞功能检测方法的选择。
