辅酶Q10对急性和慢性运动大鼠氧化应激及热休克蛋白的影响。
The effects of coenzyme Q10 on oxidative stress and heat shock proteins in rats subjected to acute and chronic exercise.
作者信息
Pala Ragip, Beyaz Fahrettin, Tuzcu Mehmet, Er Besir, Sahin Nurhan, Cinar Vedat, Sahin Kazim
出版信息
J Exerc Nutrition Biochem. 2018 Sep 30;22(3):14-20. doi: 10.20463/jenb.2018.0019.
PURPOSE
The aim of the study was to determine the effects of dietary CoQ10 on serum biochemical parameters, lipid peroxidation, and HSP expression in the liver and slow-twitch muscles (soleus and gastronemius deep portion) of exercise-trained rats.
METHODS
A total of 42 Wistar albino rats were divided into six groups: 1) Control, 2) Coenzyme Q10 (CoQ10), 3) Chronic Exercise (CE), 4) CE + CoQ10, 5) Acute Exercise (AE), and 6) AE + CoQ10. The rats were subjected to the running test 5 days a week for 6 weeks after which CoQ10 was administered via the diet. AE (running on the treadmill until the rats were exhausted) was done on the last day.
RESULTS
The results showed no significant difference in serum glucose and liver functions in any of the groups. However, CoQ10 and exercise treatment were found to lower cholesterol and triglyceride levels. Serum and muscle malondialdehyde (MDA) levels were found to be lower in the CE and CE + CoQ10 groups compared to the control group. The highest levels of HSP60, HSP70, and HSP90 in liver and muscle were found in the AE group, and the lowest levels were found in CE + CoQ10 group. CoQ10 supplementation reduced HSP expression in both CE- and AE-trained rats (P < 0.05).
CONCLUSION
The results showed that CoQ10 supplementation could reduce MDA levels, protect against oxidative damage, and regulate HSP expression in CE- and AE-trained rats. CE and CoQ10 were shown to reduce oxidative stress synergistically.
目的
本研究旨在确定膳食辅酶Q10对运动训练大鼠血清生化参数、脂质过氧化以及肝脏和慢肌（比目鱼肌和腓肠肌深部）中热休克蛋白（HSP）表达的影响。
方法
将42只Wistar白化大鼠分为六组：1）对照组，2）辅酶Q10（CoQ10）组，3）慢性运动（CE）组，4）CE + CoQ10组，5）急性运动（AE）组，6）AE + CoQ10组。大鼠每周进行5天跑步测试，持续6周，之后通过饮食给予CoQ10。AE（在跑步机上跑步直至大鼠精疲力竭）在最后一天进行。
结果
结果显示，各组大鼠的血清葡萄糖和肝功能均无显著差异。然而，发现CoQ10和运动处理可降低胆固醇和甘油三酯水平。与对照组相比，CE组和CE + CoQ10组的血清和肌肉丙二醛（MDA）水平较低。AE组肝脏和肌肉中HSP60、HSP70和HSP90的水平最高，而CE + CoQ10组最低。补充CoQ10可降低CE训练和AE训练大鼠的HSP表达（P < 0.05）。
结论
结果表明，补充CoQ10可降低MDA水平，防止氧化损伤，并调节CE训练和AE训练大鼠的HSP表达。CE和CoQ10协同作用可降低氧化应激。
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