Huang Di, Yang Yongjiang, Zhang Shuai, Su Zhuobin, Peng Tao, Wang Xiaoyuan, Zhao Yifeng, Li Shuguang
Department of Gastrointestinal Oncological Surgery, The First Affiliated Hospital of Hebei North University, Zhangjiakou, Hebei 075000, P.R. China.
Exp Ther Med. 2018 Nov;16(5):3813-3820. doi: 10.3892/etm.2018.6684. Epub 2018 Sep 3.
Regulatory T-cell density and cytotoxic T lymphocyte density are crucial in regulating antitumor immune responses. Tumor infiltration has marked therapeutic effects in gastric carcinoma, and there is evidence that chemoradiotherapy (CRT) exhibits an immune-mediated component. In the present study, the density of CD4 and CD8 cells were evaluated in post-CRT surgical samples from 68 patients with gastric cancer using immunohistochemistry. The associations between T-cell density, cytotoxic T lymphocyte density and clinical survival rate were also analyzed. Cytotoxic T lymphocyte density was associated with gastric carcinoma regression grade and regulatory T-cell density was also associated with gastric carcinoma regression grade. Of the patients who had a pathologic complete response, 84 and 76% were found to have a high CD3 and CD4 cell density, which was significantly different to patients who had a low CD3 and CD4 cell density. High cytotoxic T lymphocyte density was also associated with improved survival rates of patients with gastric cancer. In conclusion, these outcomes indicated that regulatory T-cell density and cytotoxic T lymphocyte density in the tumor microenvironment were associated with the response to neoadjuvant CRT and may represent a therapeutic target for gastric cancer.
调节性T细胞密度和细胞毒性T淋巴细胞密度在调节抗肿瘤免疫反应中至关重要。肿瘤浸润在胃癌中具有显著的治疗效果，并且有证据表明放化疗（CRT）具有免疫介导成分。在本研究中，采用免疫组织化学方法评估了68例胃癌患者CRT术后手术样本中CD4和CD8细胞的密度。还分析了T细胞密度、细胞毒性T淋巴细胞密度与临床生存率之间的关联。细胞毒性T淋巴细胞密度与胃癌消退分级相关，调节性T细胞密度也与胃癌消退分级相关。在病理完全缓解的患者中，发现84%和76%的患者CD3和CD4细胞密度较高，这与CD3和CD4细胞密度较低的患者有显著差异。高细胞毒性T淋巴细胞密度也与胃癌患者生存率的提高相关。总之，这些结果表明肿瘤微环境中的调节性T细胞密度和细胞毒性T淋巴细胞密度与新辅助CRT的反应相关，可能代表胃癌的一个治疗靶点。
