Wei Yunhai, Shen Xiaoying, Li Liqin, Cao Guoliang, Cai Xuhua, Wang Yan, Shen Hua
Department of Gastrointestinal Surgery, Huzhou Central Hospital, Huzhou, Zhejiang 313000, P.R. China.
Department of Digestion, Huzhou Central Hospital, Huzhou, Zhejiang 313000, P.R. China.
Oncol Lett. 2018 Nov;16(5):6081-6088. doi: 10.3892/ol.2018.9411. Epub 2018 Sep 6.
Gastric cancer (GC) is associated with poor patient prognosis, and so it crucial to investigate the molecular mechanisms underlying the progression of GC. The aim of the present study was to investigate the role of transmembrane-4 L6 family member 1 (TM4SF1) in the progression of GC. TM4SF1 small interfering RNA (siRNA) and TM4SF1-expressing plasmids were employed to regulate TM4SF1 expression. experiments were performed to determine the effect of TM4SF1 on the expression of apoptosis-associated molecules and determine the role of TM4SF1 in apoptosis, proliferation, migration and invasion using human GC cell lines MGC803 and MKN45. The data of the present study demonstrated that TM4SF1 may regulate the expression of apoptosis-associated molecules at the mRNA and protein levels. TM4SF1 silencing reduced B-cell lymphoma 2 (Bcl2) expression, whilst caspase-3 and Bcl2-associated X expression increased, and upregulating TM4SF1 reversed these changes in GC cells. Furthermore, TM4SF1 knockdown promoted apoptosis while inhibiting the proliferation, migration and invasion of GC cells. Rescue experiments demonstrated that TM4SF1 upregulation reversed the changes induced by transfection with TM4SF1 siRNA. In summary, TM4SF1 is an anti-apoptosis protein associated with the progression of GC. Additional experiments and clinical trials are required to confirm the possible use of TM4SF1 in tumor therapy.
胃癌（GC）与患者预后不良相关，因此研究GC进展的分子机制至关重要。本研究的目的是探讨跨膜4 L6家族成员1（TM4SF1）在GC进展中的作用。采用TM4SF1小干扰RNA（siRNA）和表达TM4SF1的质粒来调节TM4SF1的表达。利用人GC细胞系MGC803和MKN45进行实验，以确定TM4SF1对凋亡相关分子表达的影响，并确定TM4SF1在凋亡、增殖、迁移和侵袭中的作用。本研究数据表明，TM4SF1可能在mRNA和蛋白质水平调节凋亡相关分子的表达。沉默TM4SF1可降低B细胞淋巴瘤2（Bcl2）的表达，而半胱天冬酶-3和Bcl2相关X蛋白的表达增加，上调TM4SF1可逆转GC细胞中的这些变化。此外，敲低TM4SF1可促进凋亡，同时抑制GC细胞的增殖、迁移和侵袭。挽救实验表明，上调TM4SF1可逆转TM4SF1 siRNA转染诱导的变化。总之，TM4SF1是一种与GC进展相关的抗凋亡蛋白。需要进一步的实验和临床试验来证实TM4SF1在肿瘤治疗中的可能用途。
