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肝脏特异性基因表达的负调控：克隆的人视黄醇结合蛋白基因在HeLa细胞中受到抑制。

Negative control of liver-specific gene expression: cloned human retinol-binding protein gene is repressed in HeLa cells.

作者信息

Colantuoni V, Pirozzi A, Blance C, Cortese R

出版信息

EMBO J. 1987 Mar;6(3):631-6. doi: 10.1002/j.1460-2075.1987.tb04801.x.

DOI:10.1002/j.1460-2075.1987.tb04801.x
PMID:3034601
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC553444/
Abstract

It has been found that 334 bases of the 5' flanking region of the human retinol-binding protein (RBP) gene contain sufficient information to direct accurate and specific transcription in human hepatoma but not in HeLa cells. Dissection of this region reveals the existence of at least three distinct controlling elements: an enhancer, which can activate promoters in a variety of cell lines and is therefore non-tissue specific; a negative cis-acting element, which apparently binds a repressor molecule not present or non-functional in hepatoma cell lines; a promoter element. Transcription is confined to hepatoma cells only when both the negative element and the promoter element are present.

摘要

已发现人视黄醇结合蛋白（RBP）基因5'侧翼区的334个碱基包含足以在人肝癌细胞而非HeLa细胞中指导准确且特异性转录的信息。对该区域的剖析揭示了至少三种不同的调控元件的存在：一种增强子，其可在多种细胞系中激活启动子，因此是非组织特异性的；一种负性顺式作用元件，其显然结合在肝癌细胞系中不存在或无功能的阻遏分子；一种启动子元件。仅当负性元件和启动子元件都存在时，转录才局限于肝癌细胞。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9f2/553444/272ef5447b80/emboj00243-0090-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9f2/553444/576626b592f7/emboj00243-0087-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9f2/553444/242c77819b96/emboj00243-0088-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9f2/553444/ba05bb41621f/emboj00243-0088-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9f2/553444/b18381eb7c69/emboj00243-0089-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9f2/553444/272ef5447b80/emboj00243-0090-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9f2/553444/576626b592f7/emboj00243-0087-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9f2/553444/242c77819b96/emboj00243-0088-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9f2/553444/ba05bb41621f/emboj00243-0088-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9f2/553444/b18381eb7c69/emboj00243-0089-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9f2/553444/272ef5447b80/emboj00243-0090-a.jpg

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Negative regulation of transcription in eukaryotes.真核生物转录的负调控
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Host-specific activation of transcription by tandem repeats from simian virus 40 and Moloney murine sarcoma virus.来自猴病毒40和莫洛尼氏鼠肉瘤病毒的串联重复序列对转录的宿主特异性激活。
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Variety in the level of gene control in eukaryotic cells.真核细胞中基因控制水平的多样性。
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A genetic analysis of extinction: trans-dominant loci regulate expression of liver-specific traits in hepatoma hybrid cells.灭绝的遗传分析：反式显性基因座调控肝癌杂交细胞中肝脏特异性性状的表达。
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