Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, 518055, China.
College of Life Sciences, Nankai University, Tianjin, 300071, China.
Chembiochem. 2019 Feb 15;20(4):521-525. doi: 10.1002/cbic.201800438. Epub 2018 Nov 30.
Hypoxia is closely related to multiple diseases, especially in tumors, which increases the aggressiveness and drug resistance of cancer cells. Precise hypoxia imaging is of great significance for cancer diagnosis and the evaluation of therapeutic effects. A kind of hydrophobic polymer (i.e., PFPtTFPP) as an imaging probe for hypoxia with fluorene as an energy donor and an oxygen-sensitive Pt porphyrin as an energy acceptor was developed. Compact polymer dots (Pdots) with a small size were prepared by nanoprecipitation. The PFPtTFPP Pdots showed excellent hypoxia sensing in solution with high sensitivity and full reversibility. The emission intensity, quantum yields, lifetime, and single-particle brightness significantly increased under hypoxia conditions. Remarkably, hypoxia imaging in vitro and in vivo was realized, and a clear increase in brightness was observed under hypoxia conditions and in the tumor area. Excellent hypoxia imaging ability is beneficial to potential applications in cancer diagnosis.
缺氧与多种疾病密切相关，尤其是在肿瘤中，会增加癌细胞的侵袭性和耐药性。精准的缺氧成像对癌症诊断和疗效评估具有重要意义。本文设计了一种疏水性聚合物（即 PFPtTFPP）作为荧光素作为能量供体和氧敏性 Pt 卟啉作为能量受体的缺氧成像探针。通过纳米沉淀法制备了具有小尺寸的紧凑聚合物点（Pdots）。PFPtTFPP Pdots 在溶液中表现出优异的缺氧传感性能，具有高灵敏度和完全可逆性。在缺氧条件下，发射强度、量子产率、寿命和单粒子亮度显著增加。值得注意的是，实现了体外和体内的缺氧成像，在缺氧条件下和肿瘤区域观察到亮度明显增加。优异的缺氧成像能力有利于在癌症诊断中的潜在应用。
