Zhang Hongwei, Li Yaofa, Reall Tamra, Xu Yiyun, Goodman Cynthia, Saathoff Stephen, Ringbauer Joseph, Stanley David
Department of Entomology, Henan Institute of Science and Technology, Xinxiang, 453007, Henan, China.
Division of Plant Sciences, University of Missouri-Columbia, Columbia, MO, USA.
In Vitro Cell Dev Biol Anim. 2018 Dec;54(10):749-755. doi: 10.1007/s11626-018-0300-4. Epub 2018 Oct 23.
Spodoptera eridania (southern armyworm) is a polyphagous pest of many plants, including field crops, vegetables, fruits, and ornamentals. Larvae are leaf feeders, defoliating many crops in the tropics and subtropics of the western hemisphere. In this study, cell lines from S. eridania were established to support research focused on the development of advanced pest management technologies. We generated seven cell lines from larval tissues: three from nervous tissues, two from testes, and two from fat bodies. These cell lines have been passaged 18-57 times, indicating they are established lines. They are maintained in EX-CELL 420 or a combination of L15 + EX-CELL 420 media. The identities of the cell lines were confirmed by DAF-PCR and their doubling times ranged from 42 to 110 h. Microscopy indicated the presence of one or more morphologically distinct cell types in each cell line. We identified a catalase gene in all seven cell lines. HO treatment suppressed the expression of catalase and led to a reduction in catalase activity. This is the first report of cell lines established from S. eridania, and these cell lines are now available to researchers worldwide on request.
甜菜夜蛾（南方夜蛾）是一种多食性害虫，可危害多种植物，包括大田作物、蔬菜、水果和观赏植物。幼虫以叶片为食，在西半球的热带和亚热带地区使许多作物落叶。在本研究中，建立了甜菜夜蛾细胞系，以支持专注于先进害虫管理技术开发的研究。我们从幼虫组织中生成了7个细胞系：3个来自神经组织，2个来自睾丸，2个来自脂肪体。这些细胞系已传代18 - 57次，表明它们是已建立的细胞系。它们保存在EX - CELL 420或L15 + EX - CELL 420培养基的组合中。通过DAF - PCR确认了细胞系的身份，其倍增时间为42至110小时。显微镜检查表明每个细胞系中存在一种或多种形态不同的细胞类型。我们在所有7个细胞系中鉴定出一个过氧化氢酶基因。HO处理抑制了过氧化氢酶的表达并导致过氧化氢酶活性降低。这是关于从甜菜夜蛾建立细胞系的首次报道，这些细胞系现可应全球研究人员的要求提供。
