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A method for the determination of the integrity of labeled GABA used in membrane receptor binding assay.

作者信息

Cupello A, Besio G, Mainardi P

出版信息

Neurochem Res. 1987 Mar;12(3):311-3. doi: 10.1007/BF00972142.

DOI:10.1007/BF00972142
PMID:3035403
Abstract

A simple method for the determination of the proportion of true GABA within labeled "GABA" used for membrane binding assay is presented. The method is intended for the assessment of the integrity of refrigerator (+4 degrees C) stored labeled neurotransmitter. Its application allows a precise determination of the binding parameters.

摘要

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引用本文的文献

1
Chemical integrity of [3H]GABA used in binding studies.用于结合研究的[3H]GABA的化学完整性。
Neurochem Res. 1989 Jul;14(7):593-5. doi: 10.1007/BF00964866.

本文引用的文献

1
Biochemical pharmacology of the gamma-aminobutyric acid receptor/ionophore protein.γ-氨基丁酸受体/离子载体蛋白的生化药理学
Fed Proc. 1984 Oct;43(13):2773-8.
2
Gamma-aminobutyric acid binding to receptor sites in the rat central nervous system.γ-氨基丁酸与大鼠中枢神经系统中的受体位点结合。
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4802-7. doi: 10.1073/pnas.71.12.4802.
3
Influences ions, enzymes, and detergents on gamma-aminobutyric acid-receptor binding in synaptic membranes of rat brain.离子、酶和去污剂对大鼠脑突触膜中γ-氨基丁酸受体结合的影响。
Mol Pharmacol. 1977 May;13(3):442-53.
4
Endogenous inhibitor of GABA binding in mammalian brain.哺乳动物大脑中γ-氨基丁酸(GABA)结合的内源性抑制剂。
Life Sci. 1978 May 8;22(18):1653-62. doi: 10.1016/0024-3205(78)90062-0.
5
Properties of gamma-aminobutyric acid (GABA) receptor binding in rat brain synaptic membrane fractions.大鼠脑突触膜组分中γ-氨基丁酸(GABA)受体结合的特性
Brain Res. 1975 Dec 12;100(1):81-97. doi: 10.1016/0006-8993(75)90243-7.
6
Gamma-aminobutyric acid binding in mammalian brain: receptor-like specificity of sodium-independent sites.哺乳动物脑中的γ-氨基丁酸结合:非钠依赖性位点的受体样特异性
J Neurochem. 1978 Oct;31(4):933-8. doi: 10.1111/j.1471-4159.1978.tb00130.x.