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用于结合研究的[3H]GABA的化学完整性。

Chemical integrity of [3H]GABA used in binding studies.

作者信息

Balcar V J

机构信息

Department of Anatomy, University of Sydney, N.S.W., Australia.

出版信息

Neurochem Res. 1989 Jul;14(7):593-5. doi: 10.1007/BF00964866.

DOI:10.1007/BF00964866
PMID:2550828
Abstract

A method which is claimed to be able to determine the proportion of "true" GABA within radiolabeled GABA used in binding studies was tested using [3H]GABA. The method was found to be unsuitable for 3H-labeled GABA and, furthermore, both theoretical considerations and the present experimental data indicated that it could also produce misleading results with [14C]GABA.

摘要

一种据称能够确定在结合研究中使用的放射性标记γ-氨基丁酸(GABA)内“真实”GABA比例的方法,使用[³H]GABA进行了测试。结果发现该方法不适用于³H标记的GABA,此外,理论考量和当前实验数据均表明,该方法对[¹⁴C]GABA也可能产生误导性结果。

相似文献

1
Chemical integrity of [3H]GABA used in binding studies.用于结合研究的[3H]GABA的化学完整性。
Neurochem Res. 1989 Jul;14(7):593-5. doi: 10.1007/BF00964866.
2
A method for the determination of the integrity of labeled GABA used in membrane receptor binding assay.
Neurochem Res. 1987 Mar;12(3):311-3. doi: 10.1007/BF00972142.
3
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[Uptake of [14C]GABA by rat brain slices; the effect of Ca2+].[大鼠脑切片对[14C]γ-氨基丁酸的摄取;钙离子的作用]
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J Neurochem. 1986 Aug;47(2):370-4. doi: 10.1111/j.1471-4159.1986.tb04511.x.

本文引用的文献

1
Ontogeny of K(+)-stimulated release of [(3)H]GABA in rat cerebral cortex studied by a simple technique in vitro.采用一种简单的体外技术研究大鼠大脑皮质中钾离子刺激释放[³H]γ-氨基丁酸的个体发生。
Neurochem Int. 1986;8(4):573-80. doi: 10.1016/0197-0186(86)90194-4.
2
Presence of radiolabelled metabolites in release studies using [3H]gamma-aminobutyric acid.在使用[3H]γ-氨基丁酸进行的释放研究中放射性标记代谢物的存在情况。
J Neurochem. 1981 Apr;36(4):1590-3. doi: 10.1111/j.1471-4159.1981.tb00604.x.
3
A high-affinity, Na+-dependent uptake system for gamma-hydroxybutyrate in membrane vesicles prepared from rat brain.
从大鼠脑制备的膜囊泡中存在一种对γ-羟基丁酸具有高亲和力、依赖钠离子的摄取系统。
J Neurochem. 1982 Jun;38(6):1570-5. doi: 10.1111/j.1471-4159.1982.tb06634.x.
4
GABA uptake in rat central nervous system: comparison of uptake in slices and homogenates and the effects of some inhibitors.大鼠中枢神经系统中γ-氨基丁酸(GABA)的摄取:切片与匀浆摄取的比较及某些抑制剂的作用
J Neurochem. 1971 Oct;18(10):1939-50. doi: 10.1111/j.1471-4159.1971.tb09600.x.
5
Amino-oxyacetic acid: a relatively non-specific inhibitor of uptake of amino acids and amines by brain and spinal cord.氨氧基乙酸:一种相对非特异性的脑和脊髓摄取氨基酸及胺类的抑制剂。
J Neurochem. 1974 Apr;22(4):609-10. doi: 10.1111/j.1471-4159.1974.tb06904.x.
6
Effects of amino-oxyacetic acid on ( 3 H)GABA uptake by rat brain slices.
J Neurochem. 1973 Feb;20(2):431-9. doi: 10.1111/j.1471-4159.1973.tb12142.x.
7
A method for the determination of the integrity of labeled GABA used in membrane receptor binding assay.
Neurochem Res. 1987 Mar;12(3):311-3. doi: 10.1007/BF00972142.
8
GABA receptor binding in rat cerebral cortex and superior cervical ganglion in the absence of GABAergic synapses.在缺乏γ-氨基丁酸能突触的情况下,大鼠大脑皮层和颈上神经节中的γ-氨基丁酸受体结合情况。
Neurosci Lett. 1986 May 23;66(3):269-74. doi: 10.1016/0304-3940(86)90030-3.
9
Effects of GABA analogues of restricted conformation on GABA transport in astrocytes and brain cortex slices and on GABA receptor binding.受限构象的GABA类似物对星形胶质细胞和大脑皮层切片中GABA转运以及GABA受体结合的影响。
J Neurochem. 1979 Jul;33(1):181-9. doi: 10.1111/j.1471-4159.1979.tb11720.x.
10
Neurotransmitter uptake into slices of rat cerebral cortex in vitro: effect of slice size.体外大鼠大脑皮层切片中神经递质的摄取:切片大小的影响。
J Neurochem. 1976 Oct;27(4):835-9. doi: 10.1111/j.1471-4159.1976.tb05143.x.