DNA超螺旋对转录的调控：大肠杆菌gyrA和gyrB启动子的缺失分析。
Modulation of transcription by DNA supercoiling: a deletion analysis of the Escherichia coli gyrA and gyrB promoters.
作者信息
Menzel R, Gellert M
出版信息
Proc Natl Acad Sci U S A. 1987 Jun;84(12):4185-9. doi: 10.1073/pnas.84.12.4185.
Abstract
Expression of the genes determining the subunits of Escherichia coli DNA gyrase (gyrA and gyrB) is known to be induced by relaxation of the template DNA. In this paper we report a deletion analysis of the gyrA and gyrB promoter regions. We find that a DNA sequence 20 base pairs long that includes the -10 consensus region, the transcription start point, and the first few transcribed bases is responsible for the property of induction by DNA relaxation. We propose a model for relaxation-stimulated transcription in which promoter clearance is the rate-limiting step.
摘要
已知决定大肠杆菌DNA促旋酶亚基（gyrA和gyrB）的基因表达是由模板DNA的解旋诱导的。在本文中，我们报告了对gyrA和gyrB启动子区域的缺失分析。我们发现一个20个碱基对长的DNA序列，它包括-10共有区域、转录起始点和最初几个转录碱基，负责DNA解旋诱导的特性。我们提出了一个松弛刺激转录的模型，其中启动子清除是限速步骤。
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Role of the sigma subunit of Escherichia coli RNA polymerase in initiation. II. Release of sigma from ternary complexes.大肠杆菌RNA聚合酶的σ亚基在起始过程中的作用。II. σ从三元复合物中的释放
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