辣木和印楝甲醇提取物对大肠杆菌菌株的抗菌及细胞毒性作用
Antibacterial and Cytotoxic Effects of (Moringa) and (Neem) Methanolic Extracts against Strains of .
作者信息
Arévalo-Híjar Lucía, Aguilar-Luis Miguel Ángel, Caballero-García Stefany, Gonzáles-Soto Néstor, Del Valle-Mendoza Juana
机构信息
School of Odontology, Health Sciences Faculty, Universidad Peruana de Ciencias Aplicadas-UPC, Lima, Peru.
Research Center and Innovation of the Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Av. San Marcos cdra 2, Cedros de Villa, Lima, Peru.
出版信息
Int J Dent. 2018 Sep 25;2018:1071676. doi: 10.1155/2018/1071676. eCollection 2018.
OBJECTIVE
To evaluate antibacterial and cytotoxic effect of 2 methanolic extracts of and against strains of (ATCC 29212) .
METHODS
The methanolic extracts of and were prepared . The antibacterial effect of the extracts against was evaluated using the agar diffusion technique. The minimum inhibitory concentration (MIC) was determined using the microdilution method and the cytotoxicity using the cellular line MDCK.
RESULTS
The methanolic extract with the most antibacterial effect during the first 24 and 48 hours against was , evidencing a growth inhibition zone of 35.5 ± 1.05 and 44.83 ± 0.98, respectively. The MIC for both extracts was 75 g/ml. The bactericidal effect of the extract was found at a concentration of 25 g/ml and a concentration of 75 g/ml for extract.
CONCLUSIONS
In conclusion, we demonstrated that the methanolic extract of and both have an antibacterial effect against strains during the first 24 and 48 hours. None of the extracts exhibited toxicity against the cell lines under low concentrations.
目的
评估[植物名称1]和[植物名称2]的两种甲醇提取物对金黄色葡萄球菌（ATCC 29212）菌株的抗菌和细胞毒性作用。
方法
制备[植物名称1]和[植物名称2]的甲醇提取物。采用琼脂扩散技术评估提取物对金黄色葡萄球菌的抗菌作用。使用微量稀释法测定最低抑菌浓度（MIC），并使用MDCK细胞系测定细胞毒性。
结果
在最初24小时和48小时内对金黄色葡萄球菌抗菌效果最佳的甲醇提取物是[植物名称1]，其抑菌圈分别为35.5±1.05和44.83±0.98。两种提取物的MIC均为75μg/ml。发现[植物名称1]提取物在浓度为25μg/ml时具有杀菌作用，[植物名称2]提取物在浓度为75μg/ml时具有杀菌作用。
结论
总之，我们证明了[植物名称1]和[植物名称2]的甲醇提取物在最初24小时和48小时内对金黄色葡萄球菌菌株均有抗菌作用。在低浓度下，没有一种提取物对细胞系表现出毒性。
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