波斯绿茶提取物作为根管内冲洗剂对生物膜的抗菌效果

The Antibacterial Efficacy of Persian Green Tea Extract as an Intracanal Irrigant on Biofilm.

作者信息

Ramezanali Fatemeh, Samimi Shiva, Kharazifard Mohammadjavad, Afkhami Farzaneh

机构信息

Department of Endodontics, Dental School, Tehran University of Medical Sciences, International Campus, Tehran, Iran.

General Dentist, Tehran, Iran.

出版信息

Iran Endod J. 2016 Fall;11(4):304-308. doi: 10.22037/iej.2016.9.

DOI:10.22037/iej.2016.9

PMID:27790260

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5069907/

Abstract

INTRODUCTION

The aim of this study was to compare the antibacterial effect of Persian green tea extract (GTE) and 2.5% sodium hypochlorite (NaOCl) against () as an intracanal irrigant.

METHODS AND MATERIALS

Thirty freshly extracted teeth were instrumented and sectioned into mesial and distal segments. The specimens were put into wells containing 2 mL of -containing medium. After 3 weeks, the specimens were removed and divided randomly into three groups (=20). Each group was exposed to 3 mL of different irrigants for 3 min. Groups 1, 2 and 3 were irrigated with GTE, 2.5% NaOCl and normal saline, respectively. Biofilm formed in the middle third of the root canal was carved by sterile scalpel and cultured in Mueller-Hinton medium. Number of colony forming units (CFU) was counted on each plate. In addition, antimicrobial activity of the irrigants was evaluated by the agar disc diffusion test. The diameter of inhibition zone (IZ) around each irrigant was evaluated. The Kruskal-Wallis and Dunn tests were used to analysis the data.

RESULTS

While in NaOCl group no bacterial colonies were observed, the mean number of in GTE and control groups were 275±74 CFU/mL (<0.001) and 119×10±11×10 (<0.001), respectively. The mean of IZ in NaOCl and GTE groups were 24.35±0.78 and 6.9±0.87 mm, in order of appearance (<0.001). Zone of inhibition was not observed around the control group (<0.001).

CONCLUSION

This research highlighted the potential role of plant extracts in antimicrobial root canal irrigation protocol.

摘要

引言

本研究的目的是比较波斯绿茶提取物（GTE）和2.5%次氯酸钠（NaOCl）作为根管冲洗剂对（此处原文缺失相关内容）的抗菌效果。

方法与材料

30颗新鲜拔除的牙齿经过预备并切成近中及远中部分。将标本放入含有2 mL（此处原文缺失相关内容）培养基的孔中。3周后，取出标本并随机分为三组（每组n = 20）。每组用3 mL不同的冲洗剂冲洗3分钟。第1、2和3组分别用GTE、2.5% NaOCl和生理盐水冲洗。用无菌手术刀刮取根管中三分之一处形成的生物膜，并在Mueller-Hinton培养基中培养。计算每个平板上的菌落形成单位（CFU）数量。此外，通过琼脂纸片扩散试验评估冲洗剂的抗菌活性。评估每种冲洗剂周围的抑菌圈（IZ）直径。采用Kruskal-Wallis和Dunn检验分析数据。

结果

在NaOCl组未观察到细菌菌落，而GTE组和对照组的平均CFU数量分别为275±74 CFU/mL（P<0.001）和119×10±11×10（P<0.001）。NaOCl组和GTE组的IZ平均值分别为24.35±0.78和6.9±0.87 mm，呈显著性差异（P<0.001）。对照组周围未观察到抑菌圈（P<0.001）。