Punduk Zekine, Oral Onur, Ozkayin Nadir, Rahman Khalid, Varol Rana
Department of Physical Education and Sports, University of Balikesir, Balikesir 10100, Turkey.
Department of Physical Education and Sports, University of Ege, Izmir 35040, Turkey.
J Sport Health Sci. 2016 Mar;5(1):109-114. doi: 10.1016/j.jshs.2014.11.005. Epub 2015 Feb 16.
Platelet rich plasma (PRP) therapy is widely used in enhancing the recovery of skeletal muscle from injury. However, the impact of intramuscular delivery of PRP on hematologic and biochemical responses has not been fully elucidated in exercise-induced muscle damage. The purpose of this investigation the effects of intramuscular delivery of PRP on hematologic and biochemical responses and recovery strategy muscle damage induced by high intensity muscle exercise (exercise-induced muscle damage, EIMD).
Moderately active male volunteers participated in this study and were assigned to a control group (control, = 6) and PRP administration group (PRP, = 6). The subjects performed exercise with a load of 80% one repetition maximum (1RM) maximal voluntary contraction of the elbow flexors until point of exhaustion of the non-dominant arm was reached. The arms were treated with saline or autologous PRP post-24 h EIMD. Venous blood samples were obtained in the morning to establish a baseline value and 1-4 days post-exercise and were analyzed for serum ferritin, iron, iron binding capacity (IBC), creatinine kinase (CK), lactate dehydrogenase (LDH), aspartate aminotransferase (AST), and alanine aminotransferase (ALT).
The baseline levels of plasma iron, ferritin, IBC, CK, LDH, AST, and ALT were similar in both the control and PRP groups. However, 24-h following exercise a significant increase in these parameters was observed in both groups between 1 and 4 days during the recovery period. Interestingly, PRP administration decreased plasma iron levels compared to the control on the second day post-exercise. Plasma IBC increased in PRP group from Days 2 to 4 post-exercise compared to the control group whilst PRP administration had no effect on plasma ferritin, CK, AST, ALT, or LDH.
Acute exhaustive exercise increased muscle damage markers, including plasma iron, IBC, and ferritin levels, indicating muscle damage induced by exercise. PRP administration improves inflammation by reversing the increase in the iron levels post-exercise without displaying any myotoxicity and may have a role to play in the recovery of exercise-induced muscle damage.
富血小板血浆（PRP）疗法广泛用于促进骨骼肌损伤后的恢复。然而，在运动诱导的肌肉损伤中，PRP肌肉内注射对血液学和生化反应的影响尚未完全阐明。本研究的目的是探讨PRP肌肉内注射对高强度肌肉运动（运动诱导的肌肉损伤，EIMD）引起的血液学和生化反应以及恢复策略的影响。
中度活跃的男性志愿者参与本研究，分为对照组（对照组，n = 6）和PRP给药组（PRP组，n = 6）。受试者以非优势手臂80%的一次重复最大值（1RM）最大自主收缩负荷进行运动，直至达到疲劳点。在EIMD后24小时，对手臂进行生理盐水或自体PRP治疗。在早晨采集静脉血样以建立基线值，并在运动后1 - 4天进行分析，检测血清铁蛋白、铁、铁结合能力（IBC）、肌酸激酶（CK）、乳酸脱氢酶（LDH）、天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）。
对照组和PRP组血浆铁、铁蛋白、IBC、CK、LDH、AST和ALT的基线水平相似。然而，运动后24小时，两组在恢复期间的1至4天内这些参数均显著增加。有趣的是，与对照组相比，运动后第二天PRP给药降低了血浆铁水平。与对照组相比，PRP组运动后第2至4天血浆IBC升高，而PRP给药对血浆铁蛋白、CK、AST、ALT或LDH无影响。
急性力竭运动增加了肌肉损伤标志物，包括血浆铁、IBC和铁蛋白水平，表明运动诱导了肌肉损伤。PRP给药通过逆转运动后铁水平的升高改善炎症，且未显示出任何肌毒性，可能在运动诱导的肌肉损伤恢复中发挥作用。
