García-Sánchez J, Corral C, Halaihel N G, Simon M C, Alonso J L, Muzquiz J L, Ortega C, Girones O
Facultad de Veterinaria, Unidad de Patología Infecciosa y Epidemiología, Zaragoza, Spain.
Vet Microbiol. 1993 Apr;34(4):321-32. doi: 10.1016/0378-1135(93)90057-e.
A survey of rotavirus infection in a dairy herd with a history of neonatal diarrhoea was carried out. Faecal samples taken from 15 cows before and after calving as well as faeces taken from their calves daily from birth to two weeks of life were tested for rotavirus by polyacrylamide gel electrophoresis (PAGE) and compared with an ELISA and a latex agglutination commercial test. Rotavirus excretion was not detected in faeces from cows around parturition by any of the three tests. However, all of their calves shed rotaviruses during the observation period. The onset of rotavirus excretion determined by PAGE ranged from day 2 to day 8 of life (day 4.8 +/- 1.8 on average) and lasted for 4 to 7 days (5.3 +/- 1.1 days on average). Chi-square test showed a significant association (P = 0.0001) between the presence of rotavirus and the altered consistency of calves faeces. All the three tests showed similar results (overall agreement 92.5%) but discrepancies were detected mainly at the beginning or at the end of the rotavirus excretion period. Results obtained with both commercial kits closely paralleled each other and parameters other than sensitivity, specificity, diagnostic accuracy or predictive values have to be considered as selection criteria.
对一个有新生犊牛腹泻病史的奶牛群进行了轮状病毒感染调查。在产犊前后从15头奶牛采集粪便样本，以及从出生到两周龄的犊牛每天采集粪便样本，通过聚丙烯酰胺凝胶电泳（PAGE）检测轮状病毒，并与酶联免疫吸附测定（ELISA）和乳胶凝集商业检测进行比较。三种检测方法均未在分娩前后奶牛的粪便中检测到轮状病毒排泄。然而，在观察期内，它们所有的犊牛都排出了轮状病毒。通过PAGE确定的轮状病毒排泄开始时间为出生后第2天至第8天（平均为第4.8±1.8天），持续4至7天（平均为5.3±1.1天）。卡方检验显示轮状病毒的存在与犊牛粪便稠度改变之间存在显著关联（P = 0.0001）。三种检测方法结果相似（总体一致性为92.5%），但差异主要出现在轮状病毒排泄期开始或结束时。两种商业试剂盒获得的结果彼此密切平行，除敏感性、特异性、诊断准确性或预测值外的其他参数也必须作为选择标准。
