Akyuz Mehmet, Taysi Seyithan, Baysal Elif, Demir Elif, Alkis Hilal, Akan Muslum, Binici Habip, Karatas Zeynel Abidin
Department of Chemistry, Science and Art Faculty, Kilis 7 Aralık University, Kilis, Turkey.
Department of Medical Biochemistry, Gaziantep University, Medical School, Gaziantep, Turkey.
Head Neck. 2017 Oct;39(10):2027-2035. doi: 10.1002/hed.24861. Epub 2017 Jul 14.
The purpose of this study was to investigate the radioprotective effects of thymoquinone against radiation-induced damage in the salivary glands of rats exposed to total cranial gamma irradiation.
Thirty-two Sprague-Dawley rats were divided into 4 groups to test the radioprotective effectiveness of thymoquinone by intraperitoneal injection. An appropriate control group was also studied. Biochemical parameters in liver tissue of rats were determined by spectrophotometer.
Glutathione peroxidase (GSH-Px), glutathione-S-transferase (GST), total (enzymatic plus nonenzymatic) superoxide scavenger activity (TSSA), nonenzymatic superoxide scavenger activity (NSSA), and superoxide dismutase (SOD) activities were significantly decreased, whereas xanthine oxidase, nitric oxide synthase activities, malondialdehyde, nitric oxide, and peroxynitrite levels were significantly increased in the irradiation group when compared to the control and sham control groups.
Results showed that thymoquinone reduces oxidative and nitrosative stress parameters and has antioxidant effects and a free radical scavenging activity.
本研究旨在调查百里醌对接受全颅γ射线照射的大鼠唾液腺辐射诱导损伤的辐射防护作用。
将32只Sprague-Dawley大鼠分为4组，通过腹腔注射来测试百里醌的辐射防护效果。还研究了一个合适的对照组。用分光光度计测定大鼠肝脏组织中的生化参数。
与对照组和假手术对照组相比，照射组的谷胱甘肽过氧化物酶（GSH-Px）、谷胱甘肽-S-转移酶（GST）、总（酶促加非酶促）超氧化物清除活性（TSSA）、非酶促超氧化物清除活性（NSSA）和超氧化物歧化酶（SOD）活性显著降低，而黄嘌呤氧化酶、一氧化氮合酶活性、丙二醛、一氧化氮和过氧亚硝酸盐水平显著升高。
结果表明，百里醌可降低氧化和亚硝化应激参数，具有抗氧化作用和自由基清除活性。
