Bal C, Büyükşekerci M, Koca C, Ağış E R, Erdoğan S, Baran P, Gündüzöz M, Yilmaz Öh
Department of Biochemistry, Occupational Diseases Hospital, Ankara, Turkey
Department of Pharmacology, Occupational Diseases Hospital, Ankara, Turkey.
Hum Exp Toxicol. 2016 Sep;35(9):915-20. doi: 10.1177/0960327115608928. Epub 2015 Oct 1.
In this study, we aimed to investigate disulfide/thiol homeostasis in trichloroethylene (TCE) exposure. The study was carried out in 30 nonsmoker TCE-exposed workers with a variety of occupations. Additionally, 30 healthy nonsmoker volunteers were recruited as the control group. TCE exposure was determined by measuring urinary trichloroacetic acid (TCA) concentration. Median urinary TCA levels of exposed workers (20.5 mg/L) were significantly higher than control subjects (5 mg/L). Thiol and disulfide concentrations were determined using a novel automated method. Disulfide/thiol ratio was significantly higher in the exposed group (p < 0.001). Thiol/disulfide homeostasis was found to be disturbed in TCE-exposed workers. We predict that in TCE-exposed workers this disturbance can be a therapeutic target, and the efficiency of the treatment can easily be monitored by the novel method we used.
在本研究中，我们旨在调查三氯乙烯（TCE）暴露中的二硫键/硫醇稳态。该研究针对30名从事各种职业的非吸烟TCE暴露工人展开。此外，招募了30名健康非吸烟志愿者作为对照组。通过测量尿中三氯乙酸（TCA）浓度来确定TCE暴露情况。暴露工人的尿TCA水平中位数（20.5mg/L）显著高于对照组（5mg/L）。使用一种新型自动化方法测定硫醇和二硫键浓度。暴露组的二硫键/硫醇比值显著更高（p<0.001）。发现TCE暴露工人的硫醇/二硫键稳态受到干扰。我们预测，在TCE暴露工人中，这种干扰可能成为一个治疗靶点，并且可以通过我们使用的新方法轻松监测治疗效果。
