Marusić A, Kos K, Stavljenić A, Vukicević S
Department of Anatomy, School of Medicine, University of Zagreb, Croatia.
Experientia. 1993 Aug 15;49(8):693-8. doi: 10.1007/BF01923953.
Subcutaneous injection of nonspecific irritants such as magnesium silicate (talc) provokes granulomatous inflammation in the rat. Part of the acute phase response (APR) in these animals is the loss of trabecular bone at sites distant from the site of inflammation. To assess the possible involvement of vitamin D in the bone loss, we studied the development of the acute phase response in vitamin D-deprived rats. The serum APR provoked by subcutaneous inflammation in rachitic rats consisted of hypozincemia, hypercupremia, increased alkaline phosphatase activity and adrenocorticotropic hormone (ACTH) concentration, and was similar to that in control animals except for the absence of hypoferremia. Control rats with talc-induced subcutaneous inflammation also had splenomegaly and decreased total and mononuclear peripheral blood cell counts, while subcutaneous inflammation did not induce spleen changes in rachitic rats. Subcutaneous inflammation induced the loss of trabecular bone and decreased the osteoblastic cell count in tibial metaphyses in control animals. Rachitic rats had abundant osteoid on trabecular surfaces, and the number of osteoblasts and osteoclasts was comparable to that of the controls. Subcutaneous inflammation did not affect any of the bone parameters in rachitic rats. These results indicate that vitamin D plays an important role in the generation of the acute phase response during inflammation, particularly in the induction of spleen and bone cell changes. The discrepancy of the blood on one hand and bone and spleen indices of the APR on the other, indicate that they may be divergent pathways in the generation of the inflammatory response, some of which may be dependent on vitamin D.
皮下注射非特异性刺激物，如硅酸镁（滑石粉），可在大鼠体内引发肉芽肿性炎症。这些动物急性期反应（APR）的一部分是远离炎症部位的小梁骨丢失。为了评估维生素D在骨质流失中可能的作用，我们研究了维生素D缺乏大鼠急性期反应的发展情况。佝偻病大鼠皮下炎症引发的血清急性期反应包括低锌血症、高铜血症、碱性磷酸酶活性增加和促肾上腺皮质激素（ACTH）浓度升高，除了没有低铁血症外，与对照动物相似。滑石粉诱导皮下炎症的对照大鼠也有脾肿大，外周血总细胞数和单核细胞数减少，而皮下炎症在佝偻病大鼠中未引起脾脏变化。皮下炎症导致对照动物胫骨近端干骺端小梁骨丢失和成骨细胞数量减少。佝偻病大鼠小梁表面有丰富的类骨质，成骨细胞和破骨细胞数量与对照相当。皮下炎症对佝偻病大鼠的任何骨参数均无影响。这些结果表明，维生素D在炎症期间急性期反应的产生中起重要作用，特别是在脾脏和骨细胞变化的诱导中。一方面血液指标与另一方面急性期反应的骨和脾脏指标的差异表明，它们可能是炎症反应产生中的不同途径，其中一些可能依赖于维生素D。
