Bioinformatics and Entomoinformatics Lab, Department of Genetic Engineering, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, 603203, Tamil Nadu, India.
Department of Advanced Zoology and Biotechnology, Guru Nanak College, Chennai, 600042, Tamil Nadu, India.
Sci Rep. 2021 Dec 9;11(1):23738. doi: 10.1038/s41598-021-02881-0.
Oxycarenus laetus is a seed-sap sucking pest affecting a variety of crops, including cotton plants. Rising incidence and pesticide resistance by O. laetus have been reported from India and neighbouring countries. In this study, O. laetus samples were collected from Bhatinda and Coimbatore (India). Pure mtDNA was isolated and sequenced using Illumina MiSeq. Both the samples were found to be identical species (99.9%), and the complete genome was circular (15,672 bp), consisting of 13 PCGs, 2 rRNA, 23 tRNA genes, and a 962 bp control region. The mitogenome is 74.1% AT-rich, 0.11 AT, and - 0.19 GC skewed. All the genes had ATN as the start codon except cox1 (TTG), and an additional trnT was predicted. Nearly all tRNAs folded into the clover-leaf structure, except trnS1 and trnV. The intergenic space between trnH and nad4, considered as a synapomorphy of Lygaeoidea, was displaced. Two 5 bp motifs AATGA and ACCTA, two tandem repeats, and a few microsatellite sequences, were also found. The phylogenetic tree was constructed using 36 mitogenomes from 7 super-families of Hemiptera by employing rigorous bootstrapping and ML. Ours is the first study to sequence the complete mitogenome of O. laetus or any Oxycarenus species. The findings from this study would further help in the evolutionary studies of Lygaeidae.
乐食蝽是一种吸食种子汁液的害虫，会影响包括棉花在内的多种作物。据报道，印度和邻国的乐食蝽发病率上升且对农药产生了抗性。本研究从印度的 Bhatinda 和 Coimbatore 收集了乐食蝽样本。使用 Illumina MiSeq 分离并测序了纯 mtDNA。两个样本均被鉴定为相同的物种（99.9%），且完整基因组为环状（15672 bp），包含 13 个 PCGs、2 个 rRNA、23 个 tRNA 基因和一个 962 bp 的控制区。线粒体基因组富含 AT（74.1%），AT 含量为 0.11，GC 含量为-0.19。除了 cox1（TTG）外，所有基因的起始密码子均为 ATN，预测到一个额外的 trnT。除了 trnS1 和 trnV，几乎所有的 tRNA 都折叠成三叶形结构。trnH 和 nad4 之间的基因间隔区被置换，被认为是 Lygaeoidea 的一个并系同源特征。还发现了两个 5bp 基序 AATGA 和 ACCTA、两个串联重复序列和一些微卫星序列。使用 Hemiptera 的 7 个超科的 36 个线粒体基因组，通过严格的自举和 ML 构建了系统发育树。这是首次对乐食蝽或任何 Oxycarenus 物种的完整线粒体基因组进行测序的研究。本研究的结果将进一步有助于 Lygaeidae 的进化研究。
