Identification and Analysis of the Gene Family in Three Species of under Cold Stress.

The C-Repeat Binding Factor () gene family has been identified and characterized in multiple plant species, and it plays a crucial role in responding to low temperatures. Presently, only a few studies on tree species demonstrate the mechanisms and potential functions of associated with cold resistance, while our study is a novel report on the multi-aspect differences of among three tree species, compared to previous studies. In this study, genome-wide identification and analysis of the gene family in , , and were performed. The results revealed that 16 genes (five , four , and seven ) were unevenly distributed across the chromosomes, and most genes were mapped on chromosome 2 (Chr2) and chromosome 11 (Chr11). The analysis of phylogenetic relationships, gene structure, and conserved motif showed that 16 genes could be clustered into three subgroups; they all contained Motif 1 and Motif 5, and most of them only spanned one exon. The cis-acting elements analysis showed that some genes might be involved in hormone and abiotic stress responsiveness. In addition, genes exhibited tissue expression specificity. High expressions of , , , , , and , , were detected on exposure to low temperature for 3 h and 24 h. Low expressions of , , were detected under cold stress for 24 h, and and were always down-regulated under cold conditions. Taken together, comprehensive analysis will enhance our understanding of the potential functions of the genes on cold resistance, thereby providing a reference for the introduction of species in our country.