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Methylation sensitivity of the restriction enzymes FnuDII and AccII.

作者信息

Gaido M L, Strobl J S

出版信息

Arch Microbiol. 1987 Jan;146(4):338-40. doi: 10.1007/BF00410932.

DOI:10.1007/BF00410932
PMID:3034185
Abstract

The restriction enzymes FnuDII and AccII are isoschizomers of the DNA sequence 5'-CGCG-3'. We have determined that 5-methylcytidine at either cytidine position in this recognition sequence inhibits DNA cleavage by FnuDII and AccII. A third isoschizomer, ThaI was previously shown to exhibit an identical methylation sensitivity. It is remarkable that 3 restriction enzymes derived from diverse microbiological sources exhibit this identical methylation sensitivity.

摘要

相似文献

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Methylation sensitivity of the restriction enzymes FnuDII and AccII.
Arch Microbiol. 1987 Jan;146(4):338-40. doi: 10.1007/BF00410932.
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Dokl Akad Nauk SSSR. 1984;276(4):992-5.

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Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases.

本文引用的文献

1
Restriction and modification enzymes and their recognition sequences.限制酶和修饰酶及其识别序列。
Nucleic Acids Res. 1984;12 Suppl(Suppl):r167-204. doi: 10.1093/nar/12.suppl.r167.
2
Methylation of either cytosine in the recognition sequence CGCG inhibits ThaI cleavage of DNA.识别序列CGCG中任何一个胞嘧啶的甲基化都会抑制ThaI对DNA的切割。
Nucleic Acids Res. 1984 Nov 12;12(21):8073-83. doi: 10.1093/nar/12.21.8073.
3
Restriction and modification in Bacillus subtilis: sequence specificities of restriction/modification systems BsuM, BsuE, and BsuF.
位点特异性修饰对限制性核酸内切酶和DNA修饰甲基转移酶的影响。
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4
Site-specific methylation: effect on DNA modification methyltransferases and restriction endonucleases.位点特异性甲基化：对DNA修饰甲基转移酶和限制性内切酶的影响。
Nucleic Acids Res. 1991 Apr 25;19 Suppl(Suppl):2045-71. doi: 10.1093/nar/19.suppl.2045.
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Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.位点特异性甲基化对DNA修饰甲基转移酶和限制性内切酶的影响。
Nucleic Acids Res. 1992 May 11;20 Suppl(Suppl):2145-57. doi: 10.1093/nar/20.suppl.2145.
枯草芽孢杆菌中的限制与修饰：限制/修饰系统BsuM、BsuE和BsuF的序列特异性
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Site-specific deoxyribonucleases in Bacillus subtilis and other Bacillus strains.枯草芽孢杆菌及其他芽孢杆菌菌株中的位点特异性脱氧核糖核酸酶。
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Site specific endonuclease from Fusobacterium nucleatum.具核梭杆菌的位点特异性内切酶。
Nucleic Acids Res. 1979 Jan;6(1):1-15. doi: 10.1093/nar/6.1.1.
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Complete nucleotide sequence of the Escherichia coli plasmid pBR322.大肠杆菌质粒pBR322的完整核苷酸序列。
Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:77-90. doi: 10.1101/sqb.1979.043.01.013.
8
Nucleotide sequence specificity of restriction endonucleases.限制性核酸内切酶的核苷酸序列特异性
Science. 1979 Aug 3;205(4405):455-62. doi: 10.1126/science.377492.