School of Biomedical Sciences, Huaqiao University, 362021, Quanzhou, China.
Zhangzhou Health Vocational College, 363000, Zhangzhou, China.
Sci Rep. 2018 Oct 25;8(1):15831. doi: 10.1038/s41598-018-34177-1.
Rhizobacteria is an important ingredient for growth and health of medicinal herbs, and synthesis of pharmacological effective substances from it. In this study, we investigated the community structure and composition of rhizobacteria in Baphicacanthus cusia (NeeS) Bremek via 16S rRNA amplicon sequencing. We obtained an average of 3,371 and 3,730 OTUs for bulk soil and rhizosphere soil samples respectively. Beta diversity analysis suggested that the bacterial community in the rhizosphere was distinctive from that in the bulk soil, which indicates that B.cusia can specifically recruit microbes from bulk soil and host in the rhizosphere. Burkholderia was significantly enriched in the rhizosphere. Burkholderia is a potentially beneficial bacteria that has been reported to play a major role in the synthesis of indigo, which was a major effective substances in B. cusia. In addition, we found that Bacilli were depleted in the rhizosphere, which are useful for biocontrol of soil-borne diseases, and this may explain the continuous cropping obstacles in B. cusia. Our results revealed the structure and composition of bacterial diversity in B. cusia rhizosphere, and provided clues for improving the medicinal value of B. cusia in the future.
根际细菌是药用植物生长和健康的重要成分，也是合成药理有效物质的重要成分。在这项研究中，我们通过 16S rRNA 扩增子测序研究了思茅兔耳风（Baphicacanthus cusia (NeeS) Bremek）根际细菌的群落结构和组成。我们分别从根际土和非根际土中获得了平均 3371 和 3730 个 OTUs。β多样性分析表明，根际细菌群落与非根际土壤中的细菌群落明显不同，这表明 B.cusia 可以从非根际土壤中特异性招募微生物，并在根际中定植。伯克霍尔德菌在根际中显著富集。伯克霍尔德菌是一种潜在有益的细菌，已被报道在靛蓝的合成中发挥主要作用，而靛蓝是 B. cusia 的主要有效物质之一。此外，我们发现根际中芽孢杆菌减少，芽孢杆菌可用于防治土传病害，这可能解释了 B. cusia 连作障碍的原因。我们的研究结果揭示了 B. cusia 根际细菌多样性的结构和组成，为未来提高 B. cusia 的药用价值提供了线索。
