Department of Pharmacy, College of Pharmacy, Pusan National University, Busan, 46241, Republic of Korea.
J Microbiol. 2018 Dec;56(12):902-909. doi: 10.1007/s12275-018-8336-4. Epub 2018 Oct 25.
Pseudomonas aeruginosa, an opportunistic human pathogen, causes many biofilm-mediated chronic infections. In this study, biofilm structures of various clinical strains of P. aeruginosa isolated from hospitalized patients were examined and their influence on the biofilm-dispersing effects of chemicals was investigated. The clinical isolates formed structurally distinct biofilms that could be classified into three different groups: 1) mushroom-like, 2) thin flat, and 3) thick flat structures. A dispersion of these differently structured biofilms was induced using two biofilm-dispersing agents, anthranilate and sodium nitroprusside (SNP). Although both SNP and anthranilate could disperse all types of biofilms, the thick flat biofilms were dispersed less efficiently than the biofilms of other structures. This suggests that biofilm-dispersing agents have higher potency on the biofilms of porous structures than on densely packed biofilms.
铜绿假单胞菌是一种机会性病原体，可引起许多生物膜介导的慢性感染。在这项研究中，我们检查了从住院患者中分离出的各种临床铜绿假单胞菌菌株的生物膜结构，并研究了它们对化学物质的生物膜分散作用的影响。临床分离株形成了结构不同的生物膜，可以分为三种不同的类型：1）蘑菇状，2）薄而平，3）厚而平的结构。使用两种生物膜分散剂邻氨基苯甲酸和硝普酸钠（SNP）诱导这些不同结构的生物膜分散。尽管 SNP 和邻氨基苯甲酸都可以分散所有类型的生物膜，但与其他结构的生物膜相比，厚而平的生物膜分散效率较低。这表明生物膜分散剂对多孔结构的生物膜比密集堆积的生物膜具有更高的效力。
