Emami Asefesadat, Tajadini Mohamadhasan, Zeinalian Mehrdad, Keshvari Mahtab, Asgary Sedigheh
Department of Clinical Biochemistry, Shahid Sadoughi Medical University, Yazd, Iran.
Biotechnology Department, Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Interv Med Appl Sci. 2018 Mar;10(1):27-32. doi: 10.1556/1646.9.2017.41.
Paraoxonase 1 (PON1) enzyme is known enzyme with, aryl esterase, phosphatase, peroxidase, and lactonase activities. According to some studies, the activity of PON1 enzyme is decreased in type 2 diabetic patients. We analyzed the enzyme activity and its single nucleotide polymorphisms (SNPs) distribution on promoter regions (-108, -126, and -162) in type 2 diabetic patients compared with non-diabetic individuals to reveal the likely relationship between PON1 activity and its gene promoter polymorphisms.
On the whole, 98 diabetic and 104 non-diabetic individuals were examined in this study. The enzyme activity and the genotypes were studied using spectrophotometry, real-time PCR-HRM, and sequencing techniques, respectively.
There was no meaningful difference in enzyme activity between two under-studied groups (P.V = 0.671). Moreover, no meaningful difference was also seen between two groups in terms of the frequency of polymorphism -108 (P.V = 0.277). The frequencies of SNPs -126 and -162, however, showed a meaningful difference between two groups (P.V = 0.000 and P.V = 0.017, respectively).
We indicated PON1 activity could be similar in DM-2 patients and non-DM-2 individuals. The significant role of SNP -108 in PON1 activity in DM-2 patients compared with non-DM-2 individuals was confirmed in the study too. On the other hand, the role of -162 and -126 SNPs in causing diabetes cannot be easily overlook because of a meaningful difference of their distribution in understudied groups. However, they may be attributed to DM-2-associated genes.
对氧磷酶1（PON1）是一种具有芳基酯酶、磷酸酶、过氧化物酶和内酯酶活性的已知酶。根据一些研究，2型糖尿病患者中PON1酶的活性会降低。我们分析了2型糖尿病患者与非糖尿病个体相比，该酶的活性及其启动子区域（-108、-126和-162）的单核苷酸多态性（SNP）分布，以揭示PON1活性与其基因启动子多态性之间可能存在的关系。
本研究共检测了98例糖尿病患者和104例非糖尿病个体。分别采用分光光度法、实时荧光定量PCR-高分辨率熔解曲线分析（HRM）和测序技术研究酶活性和基因型。
在两个研究组之间，酶活性没有显著差异（P值=0.671）。此外，在多态性-108的频率方面，两组之间也没有显著差异（P值=0.277）。然而，SNP -126和-162的频率在两组之间显示出显著差异（P值分别为0.000和0.017）。
我们指出2型糖尿病患者和非2型糖尿病个体的PON1活性可能相似。该研究也证实了与非2型糖尿病个体相比，SNP -108在2型糖尿病患者PON1活性中的重要作用。另一方面，由于-162和-126 SNP在研究组中的分布存在显著差异，它们在导致糖尿病方面的作用不容忽视。然而，它们可能与2型糖尿病相关基因有关。
