Department of Endocrinology, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, P.R. China.
Mol Med Rep. 2018 Dec;18(6):5511-5519. doi: 10.3892/mmr.2018.9580. Epub 2018 Oct 23.
Syringaresinol‑di‑O‑β‑D‑glucoside (SOG) is a phenolic compound extracted from Polygonatum sibiricum. The present study aimed to investigate the antidiabetic effect of SOG on streptozocin (STZ)‑induced diabetic mice and determine the potential underlying mechanisms. In the present study, fasting blood glucose and organ indexes of mice were analyzed. Body weight, water intake and food intake were also recorded. Furthermore, serum fasting insulin, pancreatic insulin and pancreatic interleukin‑6 levels of mice were determined using ELISA kits to investigate the effect of SOG on the levels of insulin. Levels of total cholesterol (TC), triglyceride (TG), high‑density lipoprotein cholesterol, low‑density lipoprotein cholesterol (LDL‑C), very low‑density lipoprotein cholesterol (VLDL‑C) and free fatty acid (FFA) in the serum of mice, and levels of TC, TG and total protein in the kidney, were also determined to investigate the effects of SOG on lipid and protein metabolism in mice. Furthermore, malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase (ALP) levels, as well as total antioxidant capacity (T‑AOC), in the kidneys of mice were determined to investigate the effect of SOG on oxidative stress. Western blotting was also performed to determine the expression of proteins associated with oxidative stress. The results demonstrated that SOG (25, 50 and 75 mg/kg) induced a significant antidiabetic effect in mice. Treatment with SOG promoted insulin secretion and decreased TC, TG, LDL‑C, VLDL‑C, FFA, MDA, SOD, CAT, AST, ALT and ALP levels in the kidneys of mice, as well as kidney TC and TG levels, but increased the levels of kidney total protein and the T‑AOC in kidneys. Furthermore, SOG treatment could significantly downregulate the expressions of nitrotyrosine and transforming growth factor‑β1 in diabetic mice. Therefore, the present study indicated that SOG may exert an antidiabetic effect on STZ‑induced diabetic mice and that the mechanism of SOG may be associated with its antioxidative activity.
丁香树脂二葡萄糖苷（SOG）是从黄精中提取的酚类化合物。本研究旨在探讨 SOG 对链脲佐菌素（STZ）诱导的糖尿病小鼠的降糖作用及其潜在机制。本研究分析了小鼠的空腹血糖和器官指数。还记录了体重、饮水量和食物摄入量。此外，采用 ELISA 试剂盒测定小鼠血清空腹胰岛素、胰腺胰岛素和胰腺白细胞介素-6 水平，以研究 SOG 对胰岛素水平的影响。还测定了小鼠血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇（LDL-C）、极低密度脂蛋白胆固醇（VLDL-C）和游离脂肪酸（FFA）水平，以及肾脏 TC、TG 和总蛋白水平，以研究 SOG 对小鼠脂类和蛋白质代谢的影响。此外，还测定了小鼠肾脏丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、天门冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和碱性磷酸酶（ALP）水平以及总抗氧化能力（T-AOC），以研究 SOG 对氧化应激的影响。还进行了 Western blot 以确定与氧化应激相关的蛋白质的表达。结果表明，SOG（25、50 和 75mg/kg）在小鼠中诱导出显著的降糖作用。SOG 治疗可促进胰岛素分泌，降低小鼠肾脏 TC、TG、LDL-C、VLDL-C、FFA、MDA、SOD、CAT、AST、ALT 和 ALP 水平以及肾脏 TC 和 TG 水平，但增加肾脏总蛋白和 T-AOC 水平。此外，SOG 治疗可显著下调糖尿病小鼠肾脏中硝基酪氨酸和转化生长因子-β1 的表达。因此，本研究表明 SOG 可能对 STZ 诱导的糖尿病小鼠发挥降糖作用，其机制可能与其抗氧化活性有关。
