a Department of Sports Medicine, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
b Division of Exercise and Sport Physiology, Department of Exercise and Sport Sciences, Faculty of Sport Sciences, Hacettepe University, Ankara, Turkey.
Appl Physiol Nutr Metab. 2019 Jun;44(6):627-631. doi: 10.1139/apnm-2018-0520. Epub 2018 Oct 26.
Interleukin-6 (IL-6), IL-15, and heat shock protein 72 (Hsp72) are molecules that have significant metabolic effects on glucose and fat metabolism and a cell's stress response. The aim of this study is to determine serum levels of these molecules in runners after a long-distance trail run. Serum IL-15 levels after such endurance events have not been investigated yet. Blood samples were collected from 37 athletes (11 female, 26 male) before and after a 35-km trail run, with a total climb of 940 m. Serum was obtained from the samples, and IL-6, IL-15, and Hsp72 levels were measured from using the sandwich ELISA method. The athletes completed the race in 308.3 ± 37.4 min on average. After the race, the mean serum IL-6, IL-15, and Hsp72 concentrations increased 13.2-fold, 2.22-fold, and 1.6-fold, respectively ( < 0.001, < 0.001, and = 0.039, respectively). This is the first study to demonstrate the increase in serum IL-15 levels following an acute endurance exercise. In addition to IL-15, we report that IL-6 and soluble Hsp72 levels also increased significantly following a 35-km trail run. Since these molecules are involved in regulating glucose and fat metabolism, significant increases of IL-6, IL-15, and soluble Hsp72 may have health benefits that may be associated with long-distance trail runs, which are becoming more popular worldwide.
白细胞介素-6 (IL-6)、白细胞介素-15 (IL-15) 和热休克蛋白 72 (Hsp72) 是对葡萄糖和脂肪代谢以及细胞应激反应具有重要代谢作用的分子。本研究旨在确定长距离越野跑后这些分子在跑步者中的血清水平。尚未研究此类耐力事件后血清 IL-15 水平。从 37 名运动员（11 名女性，26 名男性）中采集血液样本，在进行 35 公里越野跑前后采集，总爬升 940 米。从样本中获得血清，并使用夹心 ELISA 法测量 IL-6、IL-15 和 Hsp72 水平。运动员的平均完赛时间为 308.3 ± 37.4 分钟。比赛后，血清 IL-6、IL-15 和 Hsp72 浓度分别增加了 13.2 倍、2.22 倍和 1.6 倍（均 < 0.001，均 < 0.001 和 = 0.039）。这是第一项证明急性耐力运动后血清 IL-15 水平增加的研究。除了 IL-15，我们还报告说，IL-6 和可溶性 Hsp72 水平在进行 35 公里越野跑后也显著增加。由于这些分子参与调节葡萄糖和脂肪代谢，因此 IL-6、IL-15 和可溶性 Hsp72 的显著增加可能具有健康益处，这些益处可能与在全球范围内越来越受欢迎的长距离越野跑有关。
