Research and Development Centre, Bharathiar University, Coimbatore, 641046, Tamil Nadu, India; Ganesh Consultancy and Analytical Services, Hebbal Industrial Area, Mysore, 570016, Karnataka, India.
Department of Chemistry, Sri Jaya Chamarajendra College of Engineering, Mysuru, 570 006, India.
Microb Pathog. 2019 Jan;126:27-39. doi: 10.1016/j.micpath.2018.10.029. Epub 2018 Oct 23.
A plant, Priva cordifolia mediated silver nanoparticle was prepared and characterized by UV-Vis spectroscopy, fourier-transform infrared spectroscopy (FT-IR), energy dispersive X-ray (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), dynamic light scattering (DLS) and x-ray diffraction (XRD) analysis. The minimum inhibitory concentration of the synthesized nanoparticle against Staphylococcus aureus was found to be 100 ± 0.80 μg/mL with 9.38 ± 0.04 mm zone of inhibition. The bactericidal activity was shown primarily due to membrane damage evident from SEM, atomic force microscopy (AFM), potassium efflux, cellular material leakage, and bio-electrochemical changes in electron transport chain data. It was also of interest to find PC@AgNPs interfering with biofilm formation by S.aureus, assessed qualitatively by SEM, confocal laser scanning microscopy (CLSM) and quantitatively by dye staining method. The bio-compatibility of PC@AgNPs was established by anti-coagulant, thrombolytic, partial thromboplastin time, thrombin-like activity and fibrinolytic activity that suggested its good maintenance of hemostatic conditions. PC@AgNPs also prevented the coagulation of rabbit plasma which as per the standard drug Dabigatran reaction was indicative of the down-regulation of virulence Coa gene expression.
采用 priva cordifolia 植物合成并对银纳米粒子进行了表征，通过紫外-可见光谱、傅里叶变换红外光谱（FT-IR）、能量色散 X 射线（EDX）、扫描电子显微镜（SEM）、透射电子显微镜（TEM）、动态光散射（DLS）和 X 射线衍射（XRD）分析。合成的纳米粒子对金黄色葡萄球菌的最小抑菌浓度（MIC）为 100±0.80μg/ml，抑菌圈直径为 9.38±0.04mm。杀菌活性主要归因于细胞膜损伤，这可以从 SEM、原子力显微镜（AFM）、钾离子外流、细胞物质泄漏以及电子传递链数据中的生物电化学变化中得到证明。有趣的是，发现 PC@AgNPs 可以干扰金黄色葡萄球菌的生物膜形成，这可以通过 SEM、共聚焦激光扫描显微镜（CLSM）和染料染色法进行定性和定量评估。PC@AgNPs 的生物相容性通过抗凝血、溶栓、部分凝血活酶时间、类凝血酶活性和纤维蛋白溶解活性来确定，这表明其对止血条件有良好的维持作用。PC@AgNPs 还可以防止兔血浆凝固，根据标准药物达比加群的反应，这表明 Coa 基因表达的毒力下调。
