Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa; Department of Food Safety and Environmental Microbiology, Centre of Expertise and Biological Diagnostic of Cameroon (CEDBCAM), Yaoundé, Cameroon.
Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa; Department of Clinical Microbiology, Centre of Expertise and Biological Diagnostic of Cameroon (CEDBCAM), Yaoundé, Cameroon.
J Glob Antimicrob Resist. 2018 Dec;15:250-251. doi: 10.1016/j.jgar.2018.10.016. Epub 2018 Oct 23.
Here we report the draft genome sequence of a methicillin-resistant Staphylococcus epidermidis strain (sequence type 59) isolated from a pooled rectal sample from pigs collected in an abattoir in South Africa.
Genomic DNA of S. epidermidis PR246B0 was sequenced using an Illumina MiSeq platform. Generated reads were de novo assembled using CLC Genomics Workbench (QIAGEN). The assembled contigs were annotated and antimicrobial resistance genes, plasmids and the sequence type were identified.
The genome comprised a circular chromosome of 2537769bp, with a G-C content of 32.32% and various antimicrobial resistance genes associated with resistance to β-lactams, fluoroquinolones, aminoglycosides, fosfomycin, macrolides, lincosamides and tetracycline. Genome analysis also revealed the presence of seven plasmid replicon types.
The genome sequence reported herein will provide useful information for a better understanding of the genetic structure of the S. epidermidis genome in Africa.
本研究报告了一株耐甲氧西林表皮葡萄球菌（序列类型 59）的基因组草图序列，该菌株从南非屠宰场采集的猪直肠混合样本中分离得到。
使用 Illumina MiSeq 平台对表皮葡萄球菌 PR246B0 的基因组 DNA 进行测序。使用 CLC Genomics Workbench（QIAGEN）对生成的读取序列进行从头组装。对组装的 contigs 进行注释，并鉴定了抗生素耐药基因、质粒和序列类型。
该基因组由一个 2537769bp 的环状染色体组成，G-C 含量为 32.32%，携带有与β-内酰胺类、氟喹诺酮类、氨基糖苷类、磷霉素、大环内酯类、林可酰胺类和四环素类抗生素耐药相关的多种抗生素耐药基因。基因组分析还显示存在七种质粒复制子类型。
本研究报告的基因组序列将为更好地了解非洲表皮葡萄球菌基因组的遗传结构提供有用信息。
