Department of Food Science and Engineering, EwhaWomans University, Seoul 120-750, Korea.
School of Systems Biomedical Science, Soongsil University, Seoul 06978, Korea.
Molecules. 2018 Oct 25;23(11):2762. doi: 10.3390/molecules23112762.
KJJ81 isolated from is an amylolytic yeast that is widely used as a microbial starter in various fermented foods. Volatile and nonvolatile metabolites of KJJ81 were investigated according to different carbon sources and cultivation times using a nontargeted metabolomic approach. Partial-least-squares discriminant analysis was applied to determine the major metabolites, which were found to be closely related to the clustering and discrimination of KJJ81 samples. Some volatile metabolites derived from phenylalanine, such as 2-phenylethanol, 2-phenylethyl acetate, and ethyl phenylacetate, were predominantly found in cultivation medium containing glucose (YPD medium). In addition, the level of 2-phenylethanol increased continuously with the cultivation time. In terms of nonvolatile metabolites, carbohydrates (mannose, arabitol, and mannitol), fatty acids (palmitic acid and stearic acid), organic acids (oxalic acid and succinic acid), and amino acids (isoleucine, serine, alanine, glutamic acid, glycine, proline, phenylalanine, and threonine) were the main contributors to KJJ81 samples cultivated in YPD medium according to cultivation time. These results show that the formation of volatile and nonvolatile metabolites of KJJ81 can be significantly affected by both the carbon sources and the cultivation time.
从 中分离出的 KJJ81 是一种糖化酵母，广泛用作各种发酵食品中的微生物发酵剂。本研究采用无靶向代谢组学方法，根据不同的碳源和培养时间研究了 KJJ81 的挥发性和非挥发性代谢产物。偏最小二乘判别分析用于确定主要代谢产物，发现其与 KJJ81 样品的聚类和区分密切相关。一些来源于苯丙氨酸的挥发性代谢产物，如 2-苯乙醇、2-苯乙酸乙酯和苯乙酸乙酯，主要存在于含有葡萄糖的培养介质（YPD 培养基）中。此外，2-苯乙醇的水平随着培养时间的延长而不断增加。就非挥发性代谢产物而言，碳水化合物（甘露糖、阿糖醇和甘露醇）、脂肪酸（棕榈酸和硬脂酸）、有机酸（草酸和琥珀酸）和氨基酸（异亮氨酸、丝氨酸、丙氨酸、谷氨酸、甘氨酸、脯氨酸、苯丙氨酸和苏氨酸）是根据培养时间，YPD 培养基中培养的 KJJ81 样品的主要贡献者。这些结果表明，KJJ81 的挥发性和非挥发性代谢产物的形成会受到碳源和培养时间的显著影响。
