College of Life Science, Nankai University, Tianjin, 300071 China.
Int J Mol Sci. 2018 Oct 25;19(11):3329. doi: 10.3390/ijms19113329.
Glutathione peroxidases (GPXs) are important enzymes in the glutathione-ascorbate cycle for catalyzing the reduction of H₂O₂ or organic hydroperoxides to water. GPXs play an essential role in plant growth and development by participating in photosynthesis, respiration, and stress tolerance. is a popular traditional Chinese medicinal plant which displays an extreme energy of tolerance to harsh alpine climate. The GPXs gene family might provide for extensively tolerance to environment stimulus. In this study, five GPX genes were isolated from . The protein amino acid sequences were analyzed by bioinformation softwares with the results that gene sequences contained three conserve cysteine residues, and the subcellular location predication were in the chloroplast, endoplasmic reticulum, or cytoplasm. Five members presented spatial and temporal specific expression with higher levels in young and green organs. And the expression patterns of in response to stresses or plant hormones were investigated by quantitative real-time PCR. In addition, the putative interaction proteins of were obtained by yeast two-hybrid with the results that could physically interact with specific proteins of multiple pathways like transcription factor, calmodulin, thioredoxin, and abscisic acid signal pathway. These results showed the regulation mechanism of were complicated and they were necessary for to adapt to the treacherous weather in highland.
谷胱甘肽过氧化物酶（GPXs）是谷胱甘肽-抗坏血酸循环中催化 H₂O₂或有机氢过氧化物还原为水的重要酶。GPXs 通过参与光合作用、呼吸作用和应激耐受，在植物生长和发育中发挥着重要作用。是一种受欢迎的传统中药植物，对高山恶劣气候表现出极强的耐受能力。GPXs 基因家族可能为广泛的环境刺激提供了。在这项研究中，从 中分离出五个 GPX 基因。通过生物信息软件对蛋白质氨基酸序列进行分析，结果表明 基因序列含有三个保守的半胱氨酸残基，亚细胞定位预测在叶绿体、内质网或细胞质中。五个 成员表现出时空特异性表达，在幼嫩和绿色器官中表达水平较高。并通过定量实时 PCR 研究了 对胁迫或植物激素的表达模式。此外，通过酵母双杂交获得了 的假定互作蛋白，结果表明 可以与转录因子、钙调蛋白、硫氧还蛋白和脱落酸信号通路等多种途径的特定蛋白发生物理相互作用。这些结果表明 的调控机制复杂，对于 适应高原恶劣天气是必要的。
