Guo Bangming, Liao Wenjuan, Wang Shusheng
Department of Neurosurgery, The First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, China.
Department of Pediatrics, The First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, China.
Transl Neurosci. 2021 Jan 20;12(1):32-39. doi: 10.1515/tnsci-2021-0005. eCollection 2021 Jan 1.
Glioblastoma multiforme (GBM) is the leading cause of death among adult brain cancer patients. Glutathione peroxidase 2 (GPX2), as a factor in oxidative stress, plays an important role in carcinogenesis. However, its role in GBM has not been well established. The study aimed to investigate the clinical significance of GPX2 with GBM prognosis.
Data of GBM and healthy individuals were retrospectively collected from oncomine, cancer cell line encyclopedia (CCLE), gene expression profiling interactive analysis (GEPIA), UALCAN, and Human Protein Atlas. GPX2 mRNA expression was first assessed across various cancer types in oncomine and cancer cell lines from CCLE. The mRNA expression of GPX2 was compared between normal and GBM tissues using GEPIA (normal = 207; GBM = 163) and UALCAN (normal = 5; GBM = 156). The GPX2 methylation was analyzed using data from UALCAN (normal = 2; GBM = 140). The prognostic value of GPX2 in GBM was explored in GEPIA and UALCAN using Kaplan-Meier method. STRING database was used to construct protein-protein interaction (PPI) network and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. Statistical significance was set as <0.05.
The current study revealed no significant differences in GPX2 expression between normal and GBM from GEPIA data ( > 0.05) and UALCAN ( = 0.257). Patients with higher GPX2 intended to have a poorer prognosis ( = 0.0089). The KEGG pathways found that chemokine-signaling pathway were the more preferred.
The findings demonstrated that GPX2 might be a potential diagnosis and prognostic indicator for GBM. Chemokine-signaling pathway may be involved in GPX2 function.
多形性胶质母细胞瘤（GBM）是成年脑癌患者死亡的主要原因。谷胱甘肽过氧化物酶2（GPX2）作为氧化应激的一个因素，在致癌过程中起重要作用。然而，其在GBM中的作用尚未完全明确。本研究旨在探讨GPX2对GBM预后的临床意义。
从Oncomine、癌细胞系百科全书（CCLE）、基因表达谱交互式分析（GEPIA）、UALCAN和人类蛋白质图谱中回顾性收集GBM和健康个体的数据。首先在Oncomine和CCLE的各种癌细胞系中评估GPX2 mRNA表达。使用GEPIA（正常 = 207；GBM = 163）和UALCAN（正常 = 5；GBM = 156）比较正常组织和GBM组织中GPX2的mRNA表达。使用UALCAN（正常 = 2；GBM = 140）的数据分析GPX2甲基化。在GEPIA和UALCAN中使用Kaplan-Meier方法探讨GPX2在GBM中的预后价值。使用STRING数据库构建蛋白质-蛋白质相互作用（PPI）网络和京都基因与基因组百科全书（KEGG）通路。设定统计学显著性为<0.05。
当前研究表明，根据GEPIA数据（>0.05）和UALCAN（=0.257），正常组织和GBM之间的GPX2表达无显著差异。GPX2水平较高的患者预后往往较差（=0.0089）。KEGG通路发现趋化因子信号通路更为相关。
研究结果表明，GPX2可能是GBM的潜在诊断和预后指标。趋化因子信号通路可能参与GPX2的功能。
