Departamento de Farmacología, Farmacia y Tecnología Farmacéutica, R+D Pharma Group (GI-1645), Facultad de Farmacia and Health Research Institute of Santiago de Compostela (IDIS), Universidade de Santiago de Compostela, E-15782 Santiago de Compostela, Spain.
FEUP-Faculdade de Engenharia, Universidade do Porto, I3S-Instituto de Investigação e Inovação em Saúde, and INEB-Instituto de Engenharia Biomédica, 4200-135 Porto, Portugal.
Carbohydr Polym. 2019 Jan 15;204:223-231. doi: 10.1016/j.carbpol.2018.10.012. Epub 2018 Oct 9.
Chronic wounds are a prevailing cause of decreased quality of life, being microbial burden a factor hindering the normal wound healing process. Aerogels are nanostructured materials with large surface area (>250 m/g) and high porosity (>96%). In this work, vancomycin-loaded chitosan aerogel beads were tested as a potential formulation to treat and prevent infections at the wound site. Processing of chitosan in the form of aerogels endowed this polysaccharide with enhanced water sorption capacity and air permeability. The morphological and textural properties of the particles were studied by image and N adsorption-desorption analysis and scanning electron microscopy. Vancomycin content and release profiles from aerogel carriers showed a fast drug release that permitted to efficiently achieve local therapeutic levels. Cell studies with fibroblasts and antimicrobial tests against S. aureus showed that the vancomycin-loaded aerogel particles were cytocompatible and effective in preventing high bacterial loads at the wound site.
慢性创面是降低生活质量的主要原因，其中微生物负担是阻碍正常创面愈合过程的一个因素。气凝胶是具有大表面积（>250 m2/g）和高孔隙率（>96%）的纳米结构材料。在这项工作中，载万古霉素壳聚糖气凝胶珠被测试为一种潜在的制剂，以治疗和预防创面感染。以气凝胶形式加工壳聚糖使这种多糖具有增强的吸水性和透气性。通过图像和 N2 吸附-解吸分析以及扫描电子显微镜研究了颗粒的形态和结构特性。气凝胶载体的万古霉素含量和释放曲线显示出快速的药物释放，能够有效地达到局部治疗水平。成纤维细胞的细胞研究和对金黄色葡萄球菌的抗菌试验表明，载万古霉素的气凝胶颗粒具有细胞相容性，并能有效防止创面高细菌负荷。
