Department of Basic Sciences, College of Science and Health Professions, King Saud Bin Abdulaziz University for Health Sciences, Jeddah, Saudi Arabia; IVF and Andrology Laboratory, Department of Obstetrics and Gynaecology, Saarland University Hospital, Building 9, Homburg/Saar 66424, Germany; Department of Medical Biochemistry and Molecular Biology, Saarland University, Building 44, 66424, Homburg/Saar, Germany.
Department of Pharmaceutical Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy and Medical Sciences, University of Petra, Amman, Jordan.
Reprod Biomed Online. 2018 Nov;37(5):581-589. doi: 10.1016/j.rbmo.2018.08.016. Epub 2018 Oct 6.
Does regular smoking affect semen quality and the levels of DNA methylation in mature human spermatozoa?
Spermatozoa from 109 men were evaluated (55 smokers and 54 non-smokers). DNA was extracted from purified spermatozoa, and DNA methylation was quantified by enzyme-linked immunosorbent assay (ELISA).
Global DNA methylation of non-smokers is significantly lower (P < 0.001) than that of smokers (4.85 ± 2.72 and 7.08 ± 1.77 ng/μl, respectively). Moreover, the mean global DNA methylation levels were significantly correlated (r = 0.22;P = 0.02) with non-condensed chromatin in the spermatozoa. Levels of non-condensed chromatin were significantly higher (P < 0.001) in smokers (29.75 ± 9.38%) compared with non-smokers (20.96 ± 11.31%). Furthermore, global sperm DNA methylation was negatively correlated with high significance (P < 0.010) with sperm: count (r = -0.27), motility (r = -0.30) and vitality (r = -0.26).
Smoking interferes with DNA methylation. Also, DNA methylation is significantly correlated with sperm parameters and sperm non-condensed chromatin. These data emphasize another detrimental effect of smoking on male fertility. DNA methylation may, therefore, be considered as a fertility marker in men.
经常吸烟是否会影响成熟人类精子的精液质量和 DNA 甲基化水平？
评估了 109 名男性的精子（55 名吸烟者和 54 名不吸烟者）。从纯化的精子中提取 DNA，并通过酶联免疫吸附试验（ELISA）定量 DNA 甲基化。
不吸烟者的全基因组 DNA 甲基化水平明显低于吸烟者（分别为 4.85±2.72 和 7.08±1.77ng/μl）。此外，精子中非凝聚染色质的平均水平与全基因组 DNA 甲基化水平呈显著相关（r=0.22；P=0.02）。与不吸烟者（20.96±11.31%）相比，吸烟者的非凝聚染色质水平明显更高（P<0.001）。此外，全精子 DNA 甲基化与精子计数（r=-0.27）、活力（r=-0.30）和活力（r=-0.26）呈显著负相关（P<0.010）。
吸烟会干扰 DNA 甲基化。此外，DNA 甲基化与精子参数和精子非凝聚染色质显著相关。这些数据强调了吸烟对男性生育力的另一种有害影响。因此，DNA 甲基化可以被认为是男性生育力的一个标志物。
