Merino O, Sánchez R, Gregorio M B, Sampaio F, Risopatrón J
Center of Biotechnology in Reproduction (CEBIOR-BIOREN), Universidad de La Frontera, Temuco, Chile; Doctoral Program in Morphological Sciences, Faculty of Medicine, Universidad de La Frontera, Temuco, Chile.
Center of Biotechnology in Reproduction (CEBIOR-BIOREN), Universidad de La Frontera, Temuco, Chile; Department Preclinical Sciences, Faculty of Medicine, Universidad de La Frontera, Temuco, Chile.
Theriogenology. 2019 Feb;125:6-11. doi: 10.1016/j.theriogenology.2018.09.030. Epub 2018 Sep 27.
Vitamin D (VD) is involved in many functions of the reproductive system male. The intake of diets high-fat and vitamin D deficiency (VD) can cause morphological and physiological changes in testis that relate to infertility in the male. However, its effects on sperm quality and in vivo fertility have been little studied. This study analyzed the effects of fat and VD on sperm quality and in vivo fertility in sperm of Sprague-Dawley rats. The rats were divided into four groups: G1: control diet with vitamin D (C/VD), G2: control diet without vitamin D (C/VD), G3: high-fat diet with vitamin D (HF/VD) and G4: high-fat diet without vitamin D (HF/VD) and were fed for 3 months. Adipose tissue weight and plasma glucose were determined. Sperm quality was analyzed for motility (MO), mitochondrial function and fertilizing capacity. The intake of a high-fat diet caused a significant increase in body weight of rats (P < 0.05). There were fat-by-VD interaction effects (P < 0.05) on MO and MMP. MO and MMP were greater (P < 0.05) in G1 (54 ± 5.5% and 60.5 ± 2.6%) than in G3 and G4 (20 ± 6.0% and 27.7 ± 3.6), whereas G2 (36.7 ± 8.9% and 30.7 ± 4.2%) was intermediate. There was no fat-by-VD interaction for fertilizing capacity. However, fertilizing capacity was greater (P > 0.05) in animals receiving control diet (70 ± 21%); than in animals receiving high-fat diet (20 ± 11%). Our results demonstrated that the high-fat diet and VD contribute to the decrease in sperm quality (MO, MMP) and consequently could decrease the fertilizing capacity.
维生素D（VD）参与男性生殖系统的多种功能。高脂饮食摄入和维生素D缺乏会导致睾丸发生形态和生理变化，这与男性不育有关。然而，其对精子质量和体内生育能力的影响研究较少。本研究分析了脂肪和VD对Sprague-Dawley大鼠精子质量和体内生育能力的影响。将大鼠分为四组：G1：含维生素D的对照饮食（C/VD），G2：不含维生素D的对照饮食（C/VD），G3：含维生素D的高脂饮食（HF/VD）和G4：不含维生素D的高脂饮食（HF/VD），喂养3个月。测定脂肪组织重量和血浆葡萄糖。分析精子质量的运动性（MO）、线粒体功能和受精能力。高脂饮食的摄入导致大鼠体重显著增加（P<0.05）。在MO和MMP方面存在脂肪与VD的交互作用（P<0.05）。G1组（54±5.5%和60.5±2.6%）的MO和MMP高于G3组和G4组（20±6.0%和27.7±3.6%），而G2组（36.7±8.9%和30.7±4.2%）处于中间水平。受精能力方面不存在脂肪与VD的交互作用。然而，接受对照饮食的动物（70±21%）的受精能力高于接受高脂饮食的动物（20±11%）（P>0.05）。我们的结果表明，高脂饮食和VD会导致精子质量（MO、MMP）下降，进而可能降低受精能力。
